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Title: Micropropagation and conservation of rare medicinal plant Wattakaka volubilis (Linn.) Stapf
Authors: Arulanandam, L John Peter
Kumar, S Ghanthi
Sowmini, M
Keywords: Wattakaka volubilis;Medicinal plant;Shoot segments;Mass propagation
Issue Date: Apr-2011
Publisher: NISCAIR-CSIR, India
Abstract: A micropropagation protocol for the important medicinal plant species Wattakaka volubilis was developed using shoot tip and nodal explants by culturing on Murashige-Skoog (MS) media fortified with 1.5 mg/L BAP in combination with 0.7 mg/L Kn that resulted in 5.3±1.4 multiple shoots per explant. Maximum shoot length observed was 5.8±1.1 cm on media supplemented with 1.5 mg/L BAP and 0.6 mg/L Kn. The in vitro raised micro shoots produced highest percentage of rooting (85%) in MS media augmented with 1.0 mg/L IBA. The hardened plantlets were acclimatized in the green house conditions and then reintroduced in the Herbal garden attached to the Centre in which 76% of the plants survived.
Page(s): 238-241
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.10(2) [April 2011]

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