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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(2) [April 2011] >
| Title: | Micropropagation and conservation of rare medicinal plant Wattakaka volubilis (Linn.) Stapf |
| Authors: | Arulanandam, L John Peter
Kumar, S Ghanthi
Sowmini, M |
| Keywords: | Wattakaka volubilis
Medicinal plant
Shoot segments
Mass propagation |
| Issue Date: | Apr-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A
micropropagation protocol for the important medicinal plant species Wattakaka volubilis was developed using
shoot tip and nodal explants by culturing on Murashige-Skoog (MS) media
fortified with 1.5 mg/L BAP in combination with 0.7 mg/L Kn that resulted in
5.3±1.4 multiple shoots per explant. Maximum shoot length observed was 5.8±1.1
cm on media supplemented with 1.5 mg/L BAP and 0.6 mg/L Kn. The in vitro raised
micro shoots produced highest percentage of rooting (85%) in MS media augmented
with 1.0 mg/L IBA. The hardened plantlets were acclimatized in the green house
conditions and then reintroduced in the Herbal garden attached to the Centre in
which 76% of the plants survived. |
| Page(s): | 238-241 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(2) [April 2011]
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