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IJBT Vol.10(2) [April 2011] >


Title: Kappa casein gene polymorphism in Zalawadi goats
Authors: Patel, S B
Pande, A M
Rank, D N
Arya, J S
Jacob, Ninan
Keywords: CSN3 gene
HaeIII
PCR-RFLP
Polymorphism
Zalawadi goat
Issue Date: Apr-2011
Publisher: NISCAIR-CSIR, India
Abstract: Kappa casein protein variants were analysed in blood samples of 50 unrelated Zalawadi goat breeds by PCR-RFLP techniques with HaeIII restriction enzyme. Two primers (5΄-TCC CAA TGT TGT ACT TTC TTA ACA TC-3΄) and (5΄-GCG TTG TCC TCT TTG ATG TCT CCT TAG-3΄) were used as forward and reverse primers, respectively for the amplification of CSN3 gene. The resultant PCR product observed at 645 bp was digested with restriction endonuclease HaeIII, and the resultant fragments of 416 bp and 229 bp after separation by electrophoresis were visualized and documented by gel documentation system, which indicated the presence of Kappa casein AA genotype in population. The band sizes were compared with molecular size marker and found to be of the same size in all the 50 samples.
Page(s): 235-237
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.10(2) [April 2011]

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