|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(2) [April 2011] >
| Title: | Kappa casein gene polymorphism in Zalawadi goats |
| Authors: | Patel, S B
Pande, A M
Rank, D N
Arya, J S
Jacob, Ninan |
| Keywords: | CSN3 gene
HaeIII
PCR-RFLP
Polymorphism
Zalawadi goat |
| Issue Date: | Apr-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Kappa casein protein
variants were analysed in blood samples of 50 unrelated Zalawadi goat breeds by
PCR-RFLP techniques with HaeIII restriction enzyme. Two primers (5΄-TCC
CAA TGT TGT ACT TTC TTA ACA TC-3΄) and (5΄-GCG
TTG TCC TCT TTG ATG TCT CCT TAG-3΄) were used as
forward and reverse primers, respectively for the amplification of CSN3 gene. The resultant PCR product observed
at 645 bp was digested with restriction endonuclease HaeIII, and the
resultant fragments of 416 bp and 229 bp after separation by electrophoresis
were visualized and documented by gel documentation system, which indicated the
presence of Kappa casein AA genotype in population. The band sizes were compared
with molecular size marker and found to be of the same size in all the 50
samples. |
| Page(s): | 235-237 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(2) [April 2011]
|
|
