| Abstract: | Murrah buffaloes (n=25) were used to explore
the genetic polymorphism in DRB3 exon 2 by PCR-RFLP and to find out the
association with mastitis. The gDNA was isolated from whole blood samples as per
standard protocol and a 304 bp PCR was amplified. When 304 bp product of exon 2
of DRB3 gene was digested with RsaI, it revealed 10 alleles (a, b, c, f, g, i,
l, o, s & t) with 12 different genotypes (a/a, b/b, c/c, f/f, i/i, o/o,
s/s, t/t, o/g, i/f, l/t, & i/s). HaeIII revealed 5 alleles (a, b, d,
e & i) and
8 genotypes (a/a, e/e, d/d, b/b, a/e, a/b, a/d & b/i). On digestion with
PstI, only 4 alleles (x, y, z & s) with 7 different genotypes (x/x, y/y,
z/z, x/y, x/z, s/z & y/z) were observed. The following genotypes a/a, c/c,
and f/f (RsaI); b/b, d/d, e/e, and b/I (HaeIII); x/x, y/y, x/y, and y/z (PstI)
were observed only in healthy animals. These results revealed that the DRB3.2
gene was more polymorphic in the healthy animals than mastitis cases. |
