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Title: Polymorphism in DRB3 exon 2 by PCR-RFLP and its association with mastitis in Murrah buffaloes
Authors: Kumar, Sudhir
Sangwan, M L
Ahlawat, Sonika
Barwar, Anurag
Keywords: BuLA-DRB3.2;PCR-RFLP;Murrah buffalo;Mastitis
Issue Date: Apr-2011
Publisher: NISCAIR-CSIR, India
Abstract: Murrah buffaloes (n=25) were used to explore the genetic polymorphism in DRB3 exon 2 by PCR-RFLP and to find out the association with mastitis. The gDNA was isolated from whole blood samples as per standard protocol and a 304 bp PCR was amplified. When 304 bp product of exon 2 of DRB3 gene was digested with RsaI, it revealed 10 alleles (a, b, c, f, g, i, l, o, s & t) with 12 different genotypes (a/a, b/b, c/c, f/f, i/i, o/o, s/s, t/t, o/g, i/f, l/t, & i/s). HaeIII revealed 5 alleles (a, b, d, e & i) and 8 genotypes (a/a, e/e, d/d, b/b, a/e, a/b, a/d & b/i). On digestion with PstI, only 4 alleles (x, y, z & s) with 7 different genotypes (x/x, y/y, z/z, x/y, x/z, s/z & y/z) were observed. The following genotypes a/a, c/c, and f/f (RsaI); b/b, d/d, e/e, and b/I (HaeIII); x/x, y/y, x/y, and y/z (PstI) were observed only in healthy animals. These results revealed that the DRB3.2 gene was more polymorphic in the healthy animals than mastitis cases.
Page(s): 232-234
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.10(2) [April 2011]

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