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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(2) [April 2011] >
| Title: | Establishment of a rapid multiplication protocol of Coleus forskohlii Briq. and in vitro conservation by reduced growth |
| Authors: | Dube, Pratibha Gangopadhyay, Moumita Dewanjee, Saikat Ali, M Nasim |
| Keywords: | Coleus forskohlii in vitro conservation Mannitol Micropropagation Slow growth |
| Issue Date: | Apr-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Coleus forskohlli Briq., an endangered medicinal plant, has been successfully micropropagated under in vitro conditions. Best adventitious shoots were developed in solid Murashige and Skoog’s medium supplemented with 1.5 mg L-l 6-benzylaminopurine, while best rhizogenesis was achieved without growth regulator. The second event of success was in vitro conservation of the species by slow growth technique employing osmotic regulators (sorbitol and mannitol). 3 M mannitol showed best performance for reducing growth. This study elucidated an efficient method of ex situ conservation of germplasm and sustainable management of this medicinally important plant. |
| Page(s): | 228-231 |
| CC License: | CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(2) [April 2011]
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