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|Title:||Establishment of a rapid multiplication protocol of <i>Coleus forskohlii</i> Briq. and <i>in vitro</i> conservation by reduced growth|
Ali, M Nasim
<i style="">in vitro</i> conservation
|Abstract:||<i style="">Coleus</i> <i style="">forskohlli </i>Briq., an endangered medicinal plant, has been successfully micropropagated under <i style="">in vitro</i> conditions. Best adventitious shoots were developed in solid Murashige and Skoog’s medium supplemented with 1.5 mg L<sup>-l</sup> 6-benzylaminopurine, while best rhizogenesis was achieved without growth regulator. The second event of success was <i>in vitro</i> conservation of the species by slow growth technique employing osmotic regulators (sorbitol and mannitol). 3 <i style="">M</i> mannitol showed best performance for reducing growth. This study elucidated an efficient method of <i>ex situ</i> conservation of germplasm and sustainable management of this medicinally important plant.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.10(2) [April 2011]|
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