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Title: Establishment of a rapid multiplication protocol of <i>Coleus forskohlii</i> Briq. and <i>in vitro</i> conservation by reduced growth
Authors: Dube, Pratibha
Gangopadhyay, Moumita
Dewanjee, Saikat
Ali, M Nasim
Keywords: <i>Coleus</i> <i>forskohlii</i>
<i style="">in vitro</i> conservation
Slow growth
Issue Date: Apr-2011
Publisher: NISCAIR-CSIR, India
Abstract: <i style="">Coleus</i> <i style="">forskohlli </i>Briq., an endangered medicinal plant, has been successfully micropropagated under <i style="">in vitro</i> conditions. Best adventitious shoots were developed in solid Murashige and Skoog’s medium supplemented with 1.5 mg L<sup>-l</sup> 6-benzylaminopurine, while best rhizogenesis was achieved without growth regulator. The second event of success was <i>in vitro</i> conservation of the species by slow growth technique employing osmotic regulators (sorbitol and mannitol). 3 <i style="">M</i> mannitol showed best performance for reducing growth. This study elucidated an efficient method of <i>ex situ</i> conservation of germplasm and sustainable management of this medicinally important plant.
Description: 228-231
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.10(2) [April 2011]

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