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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(2) [April 2011] >
| Title: | Immobilization of Arthrobacter sarcosine oxidase onto alkylamine and arylamine glass and its application in serum sarcosine determination |
| Authors: | Pundir, C S
Chauhan, Nidhi
Kumari, Garima
Vandana |
| Keywords: | Arthrobacte
Sarcosine
Sarcosine oxidase
Alkylamine glass
Arylamine glass
Immobilization
Serum |
| Issue Date: | Apr-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Commercial sarcosine oxidase (Sarcosine: O2
oxidoreductase, EC 1.5.3.1)
from Arthrobacter sp. has been
immobilized onto alkyl and arylamine glass-beads through glutaraldehyde cross
linking and diazotization with a conjugation yield of
0.86 mg/g & 0.91 mg/g support and 75.8% & 84.6% retention of initial
activity of free enzyme, respectively. The enzyme showed decrease in optimum pH and Km for sarcosine but
increase in temperature for maximum activity and time for linearity after
immobilization. The glass beads were employed for colorimetric determination of
sarcosine in serum. The minimum detection limit of the method was 0.05 mM.
The glass beads were reused 100-times without considerable loss of activity,
when stored in distilled water at 4°C
for a period of two months. |
| Page(s): | 219-223 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(2) [April 2011]
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