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IJBT Vol.10(2) [April 2011] >


Title: Immobilization of Arthrobacter sarcosine oxidase onto alkylamine and arylamine glass and its application in serum sarcosine determination
Authors: Pundir, C S
Chauhan, Nidhi
Kumari, Garima
Vandana
Keywords: Arthrobacte
Sarcosine
Sarcosine oxidase
Alkylamine glass
Arylamine glass
Immobilization
Serum
Issue Date: Apr-2011
Publisher: NISCAIR-CSIR, India
Abstract: Commercial sarcosine oxidase (Sarcosine: O2 oxidoreductase, EC 1.5.3.1) from Arthrobacter sp. has been immobilized onto alkyl and arylamine glass-beads through glutaraldehyde cross linking and diazotization with a conjugation yield of 0.86 mg/g & 0.91 mg/g support and 75.8% & 84.6% retention of initial activity of free enzyme, respectively. The enzyme showed decrease in optimum pH and Km for sarcosine but increase in temperature for maximum activity and time for linearity after immobilization. The glass beads were employed for colorimetric determination of sarcosine in serum. The minimum detection limit of the method was 0.05 mM. The glass beads were reused 100-times without considerable loss of activity, when stored in distilled water at 4°C for a period of two months.
Page(s): 219-223
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.10(2) [April 2011]

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