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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(2) [April 2011] >
| Title: | Molecular diagnostics of human pathogenic Aspergillus species |
| Authors: | Gehlot, Praveen
Purohit, D K
Singh, S K |
| Keywords: | Aspergillu
Aspergillosis
Genetic markers
RAPD
5.8S rRNA gene sequencing |
| Issue Date: | Apr-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Eight species
of Aspergillus (A. hiratsukae, A. flavus, A. fumigatus, A. terreus, A. niger, A.
sydowii, A. clavatus and A. flavipes)
were found associated with clinical samples causing human diseases. The nonspecific
symptoms and lack of rapid diagnostics is a major problem in treating patients
suffering from invasive aspergillosis. Molecular markers based on RAPD primers
and diversity in ITS region of 5.8S rRNA gene developed for rapid diagnosis
facilitated early treatment of patient suffering from aspergillosis related
disorders. Direct sequencing of amplified ITS products revealed significant
single nucleotide polymorphism, which facilitated identification of pathogenic Aspergillus species associated with aspergillosis.
The complete sequences of eight Aspergillus
species were submitted to NCBI database, USA, which were assigned new Gen
accession numbers EU515147 to EU515154 and now available in public domain for
comparison. |
| Page(s): | 207-211 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(2) [April 2011]
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