Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/11449
Title: Molecular diagnostics of human pathogenic <i style="">Aspergillus</i> species
Authors: Gehlot, Praveen
Purohit, D K
Singh, S K
Keywords: <i style="">Aspergillu</i>
Aspergillosis
Genetic markers
RAPD
5.8S rRNA gene sequencing
Issue Date: Apr-2011
Publisher: NISCAIR-CSIR, India
Abstract: Eight species of <i style="">Aspergillus </i>(<i style="">A. hiratsukae, A. flavus, A. fumigatus, A. terreus, A. niger, A. sydowii, A. clavatus </i>and<i style=""> A. flavipes</i>) were found associated with clinical samples causing human diseases. The nonspecific symptoms and lack of rapid diagnostics is a major problem in treating patients suffering from invasive aspergillosis. Molecular markers based on RAPD primers and diversity in ITS region of 5.8S rRNA gene developed for rapid diagnosis facilitated early treatment of patient suffering from aspergillosis related disorders. Direct sequencing of amplified ITS products revealed significant single nucleotide polymorphism, which facilitated identification of pathogenic <i style="">Aspergillus</i> species associated with aspergillosis. The complete sequences of eight <i style="">Aspergillus</i> species were submitted to NCBI database, USA, which were assigned new Gen accession numbers EU515147 to EU515154 and now available in public domain for comparison.
Description: 207-211
URI: http://hdl.handle.net/123456789/11449
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.10(2) [April 2011]

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