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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(2) [April 2011] >
| Title: | Homology modeling deduced 3D structure of the Cry1Ab22 toxin |
| Authors: | Kashyap, S
Singh, B D
Amla, D V |
| Keywords: | 3D structure
Homology modelling
Cry1Ab22
Bacillus thuringiensis |
| Issue Date: | Apr-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: |  -Endotoxin Cry1Ab22 is produced by Bacillus
thuringiensis BtS2491Ab. The toxic spectrum of this protein is reported to
span Lepidopteron and Dipteran. Here, we predict the theoretical structural
model of newly reported Cry1Ab22 toxin by homology modeling method on the
structure of the Cry1Aa toxin. Proposed model resembles the target by
sharing common three dimensional, three domain structure. The main differences
being located in the length of loops, absence of helixes ( 7b, 10a, 10b,11a)
and presence of additional components  21, 9b). Few of the components like 9a,9b and 12a are positioned spatially at different locations. A better
understanding of the 3D structure will be helpful in designing the domain
swapping and mutagenesis experiments aimed at improving toxicity, and will lead
to a deeper understanding of the common mechanism of toxins. |
| Page(s): | 202-206 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(2) [April 2011]
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