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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.49 [2011] >
IJEB Vol.49(04) [April 2011] >
| Title: | Comparative study of 6-APA production by free and agar immobilized bacteria in nutrient broth culture |
| Authors: | Dolui, A K
Das, S |
| Keywords: | 6- Aminopenicillanic acid
Bioconversion
HPLC assessment
Penicillin G
Penicillin G acylase |
| Issue Date: | Apr-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | In the present study different bacterial samples were
isolated from soil of different places of Dibrugarh and screened for
biotransformation ability to produce 6-Aminopenicillanic acid. Among ten
isolated bacterial samples, three gram positive bacterial samples designated as
AKDD-2, AKDD-4 and AKDD-6 showed the production of 6-APA from penicillin G.
Assessment of production of 6-APA after incubation in penicillin G (2 mg/ml) by
three different samples separately in free and agar immobilization state was
done by HPLC analysis. Reusability of immobilized
cells was found successful up to 14 days. |
| Page(s): | 289-292 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.49(04) [April 2011]
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