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IJEB Vol.49(04) [April 2011] >

Title: Comparative study of 6-APA production by free and agar immobilized bacteria in nutrient broth culture
Authors: Dolui, A K
Das, S
Keywords: 6- Aminopenicillanic acid
HPLC assessment
Penicillin G
Penicillin G acylase
Issue Date: Apr-2011
Publisher: NISCAIR-CSIR, India
Abstract: In the present study different bacterial samples were isolated from soil of different places of Dibrugarh and screened for biotransformation ability to produce 6-Aminopenicillanic acid. Among ten isolated bacterial samples, three gram positive bacterial samples designated as AKDD-2, AKDD-4 and AKDD-6 showed the production of 6-APA from penicillin G. Assessment of production of 6-APA after incubation in penicillin G (2 mg/ml) by three different samples separately in free and agar immobilization state was done by HPLC analysis. Reusability of immobilized cells was found successful up to 14 days.
Page(s): 289-292
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.49(04) [April 2011]

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