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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.49 [2011] >
IJEB Vol.49(04) [April 2011] >
| Title: | Effect of anti-mosquito midgut antibodies on development of malaria parasite, Plasmodium vivax and fecundity in vector mosquito Anopheles culicifacies (Diptera: culicidae) |
| Authors: | Chugh, Manoj
Adak, T
Sehrawat, Neelam
Gakhar, S K |
| Keywords: | Anti-mosquito midgut antibodies
Anopheles culicifacies
Plasmodium vivax |
| Issue Date: | Apr-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The effect of anti-mosquito-midgut antibodies on the development of the
malaria parasite, P.vivax was studied
by feeding the vector mosquito, An.
culicifacies with infected blood supplemented with serum from immunized
rabbits. In order to get antisera, rabbits were immunized with midgut proteins
of three siblings species of Anopheles
culicifacies, reported to exhibit differential vectorial capacity. The mosquitoes that ingested anti-midgut
antibodies along with infectious parasites had significantly fewer oocysts
compared to the control group of mosquitoes. The immunized rabbits generated
high titer of antibodies. Their cross reactivity amongst various tissues of the
same species and with other sibling species was also determined. Immunogenic
polypeptides expressed in the midgut of glucose or blood fed An. culicifacies sibling species were
identified by Western blotting. One immunogenic polypeptide of 62 kDa was
exclusively present in the midgut of species A. Similarly, three polypeptides
of 97, 94 and 58 kDa and one polypeptide of 23 kDa were present exclusively in
species B and C respectively. Immunoelectron microscopy revealed the
localization of these antigens on baso-lateral membrane and microvilli. The
effects of anti-mosquito midgut antibodies on fecundity, longevity, mortality
and engorgement of mosquitoes were studied. Fecundity was also reduced
significantly. These observations open an avenue for research toward the
development of a vector-based malaria parasite transmission-blocking vaccine. |
| Page(s): | 245-253 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.49(04) [April 2011]
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