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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Geo-Marine Sciences (IJMS) >
IJMS Vol.40 [2011] >
IJMS Vol.40(1) [February 2011] >
| Title: | Isolation and characterization of 37 kDa heparinase from the purple fluid of Dolabella auricularia |
| Authors: | Abirami, P.
Arumugam, M.
Ajithkumar, T.T.
Balasubramanian, T. |
| Keywords: | Nudibranch heparinase
Dolabella auricularia
Purple fluid
DEAE-cellulose |
| Issue Date: | Feb-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Present study was to
explore the heparinase and antiglycosidase activity of the purple fluid of Dolabella auricularia. Heparinase
activity was found as 56.42 IU/ml, further the activity was confirmed by APPT
test by the reduction of coagulation time with the addition of purple fluid of
about 7±0.2. This purple fluid was dialyzed and purified by anion exchange column
(DEAE-cellulose). Amount of protein in crude and purified sample was 2mg/ml and
7.6mg/ml. Apparent molecular weight of the purified heparinase was reveled by
SDS-PAGE as 37 kDa. Effects of various factors such as temperature and pH were
determined and recorded the maximum activity at 30ºC-50ºC and 7 respectively.
Antibacterial activity was also observed against four pathogens and activity
was observed maximum only in P.
aeruginosa, S. aureus. Antiglycosidase
activity exhibits about 120.9IU/ml which has been reduced when compared to that
standard. Present study suggests the purple fluid of D. auricularia has immense uses in pharmaceutical industries. |
| Page(s): | 112-116 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1033 (Online); 0379-5136 (Print) |
| Source: | IJMS Vol.40(1) [February 2011]
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