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Title: Isolation and characterization of 37 kDa heparinase from the purple fluid of Dolabella auricularia
Authors: Abirami, P.
Arumugam, M.
Ajithkumar, T.T.
Balasubramanian, T.
Keywords: Nudibranch heparinase;Dolabella auricularia;Purple fluid;DEAE-cellulose
Issue Date: Feb-2011
Publisher: NISCAIR-CSIR, India
Abstract: Present study was to explore the heparinase and antiglycosidase activity of the purple fluid of Dolabella auricularia. Heparinase activity was found as 56.42 IU/ml, further the activity was confirmed by APPT test by the reduction of coagulation time with the addition of purple fluid of about 7±0.2. This purple fluid was dialyzed and purified by anion exchange column (DEAE-cellulose). Amount of protein in crude and purified sample was 2mg/ml and 7.6mg/ml. Apparent molecular weight of the purified heparinase was reveled by SDS-PAGE as 37 kDa. Effects of various factors such as temperature and pH were determined and recorded the maximum activity at 30ºC-50ºC and 7 respectively. Antibacterial activity was also observed against four pathogens and activity was observed maximum only in P. aeruginosa, S. aureus. Antiglycosidase activity exhibits about 120.9IU/ml which has been reduced when compared to that standard. Present study suggests the purple fluid of D. auricularia has immense uses in pharmaceutical industries.
Page(s): 112-116
ISSN: 0975-1033 (Online); 0379-5136 (Print)
Appears in Collections:IJMS Vol.40(1) [February 2011]

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