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Title: Design and Optimization of Novel Immuno-dipstick Test for Detection of Teliospores of Kamal Bunt (Tilletia indica) of Wheat
Authors: Kesari, Ravi
Kumar, Anil
Keywords: Karnal bunt
Poly clonal antibodies
Immuno-dipstick test
Issue Date: Oct-2003
Publisher: NISCAIR-CSIR, India
Abstract: An immuno-dipstick test was developed for detection of teliospores of Karnal bunt using crude and DE-52 purified anti-teliospore antibodies. For development of this test, intact teliospores and solubilized teliosporic antigen were coated on nitrocellulose pad of dipsticks. On observation of the nitrocellulose pad under a stereo zoom microscope, the intact teliospores were found stuck on the pad after coating but got washed away with first washing. The positive tests after immunoprocessing of dipsticks indicate that surface antigen may tear off from teliospores and remains bound to nitrocellulose pad. Standardization and optimization of the test was done following antigen concentration kinetics and antibody dilution analysis as well as antibody incubation time. The test was also performed for checking cross-reactivity with other fungal pathogens. DE-52 purified antiserum gave partial reactivity with spores of only Tilletia barclayana and Puccinia recondita. The sensitivity of test was found to be 5 teliospores and 10 ng for solubilized teliosporic antigen.
Description: 603-606
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.02(4) [October 2003]

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