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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.02 [2003] >
IJBT Vol.02(4) [October 2003] >
| Title: | Design and Optimization of Novel Immuno-dipstick Test for Detection of Teliospores of Kamal Bunt (Tilletia indica) of Wheat |
| Authors: | Kesari, Ravi
Kumar, Anil |
| Keywords: | Karnal bunt
Teliospore
Poly clonal antibodies
Immunodetection
Immuno-dipstick test |
| Issue Date: | Oct-2003 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | An
immuno-dipstick test was developed for detection of teliospores of Karnal bunt
using crude and DE-52
purified
anti-teliospore antibodies. For development of this test, intact teliospores
and solubilized teliosporic antigen
were
coated on nitrocellulose pad of dipsticks. On observation of the nitrocellulose
pad under a stereo zoom
microscope,
the intact teliospores were found stuck on the pad after coating but got washed
away with first washing.
The
positive tests after immunoprocessing of dipsticks indicate that surface
antigen may tear off from teliospores and
remains
bound to nitrocellulose pad. Standardization and optimization of the test was
done following antigen
concentration
kinetics and antibody dilution analysis as well as antibody incubation time.
The test was also
performed
for checking cross-reactivity with other fungal pathogens. DE-52 purified
antiserum gave partial
reactivity
with spores of only Tilletia barclayana and Puccinia recondita. The
sensitivity of test was found to be 5
teliospores
and 10 ng for solubilized teliosporic antigen. |
| Page(s): | 603-606 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.02(4) [October 2003]
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