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Title: Caspase-3 like Protein in Wheat- Tilletia indica Dual Culture System as Potential Biomarker for Host Resistance to Kamal Bunt
Authors: Sethy, Niroj Kumar
Pandey, Dinesh
Singh, U S
Kumar, Anil
Keywords: Wheat;Dual culture;Sporidia;Karnal bunt;Tilletia indica;Resistance;Bio-marker;Caspase-3;P53;Immunoassay
Issue Date: Oct-2003
Publisher: NISCAIR-CSIR, India
Abstract: Immuno-histochemical staining of dual co-cultured calli of wheat-Tilletia indica having resistant and susceptible line using anti-mycelial and anti-teliospore polyclonal antibodies revealed the differential resistance response as indicated by pathogen invasion, mycelial colonization, plasmolysis and immunolocalization of chlamydospore like structures. The p53and caspases, crucial proteins of cell death pathways were detected by immunological procedures using heterologous immunoprobes in fungal colonized wheat calli. There was no difference in p53expression in resistant and susceptible lines at different days after inoculation (DAI) of T. indica on calli raised from mature embryos. The expression of p53started 14 DAI, increased steadily up to 21 DAI and became constant at 30 DAI showing the saturation of expression level. Studies on the expression of caspases revealed the absence of caspase-I like protein at different stages of growth. The caspase-3 like protein was induced at 3 DAI in both hosts and its expression steadily increased at 14 DAI and reached a plateau at 21 DAI. A remarkable difference in the level of expression of this protein between resistant and susceptible co-cultured calli was observed. Differential activation of caspase-3 like protein was observed in 10 hosts and non-hosts. It was found to be correlated with the degree of susceptibility and the expression of resistance at cellular level. Hence, it could be used as a potential biomarker for identification of Karnal Bunt resistance sources.
Page(s): 596-602
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.02(4) [October 2003]

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