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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.02 [2003] >
IJBT Vol.02(4) [October 2003] >
| Title: | Evaluation of Multiplex Reverse Transcription Polymerase Chain Reaction (RT-PCR) for Simultaneous Detection of Potato Viruses and Strains |
| Authors: | Verma, Yogita
Sood, Shivali
Ahlawat, Y S
Khurana, S M Paul
Nie, X
Singh, R P |
| Keywords: | Detection of viruses
Multiplex RT-PCR
Potato viruses
Strains |
| Issue Date: | Oct-2003 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A
multiplex reverse transcription polymerase chain reaction (RT-PCR) protocol was
evaluated for the
simultaneous
detection of Potato leaf roll virus (PLRV), Potato virus X (PVX)
and Potato Virus Y (PVY). The
multiplex
RT-PCR detection of viruses was equally efficient whether RNAs were extracted
using commercial kits or
a
sodium sulphite based nucleic acid extraction procedure. cDNAs were prepared
either using a common primer
(oligo
dT) or specific antisense primer followed by specific primer pairs for PCR. The
multiplex RT-PCR separation
of
strains of PVY was accomplished by using a competitive multiplex RT-PCR (with
one antisense and two sense
primers).
The multi virus or multistrain detection approaches described here have
potential application to other
crop-virus combinations. |
| Page(s): | 587-590 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.02(4) [October 2003]
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