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Title: Evaluation of Multiplex Reverse Transcription Polymerase Chain Reaction (RT-PCR) for Simultaneous Detection of Potato Viruses and Strains
Authors: Verma, Yogita
Sood, Shivali
Ahlawat, Y S
Khurana, S M Paul
Nie, X
Singh, R P
Keywords: Detection of viruses;Multiplex RT-PCR;Potato viruses;Strains
Issue Date: Oct-2003
Publisher: NISCAIR-CSIR, India
Abstract: A multiplex reverse transcription polymerase chain reaction (RT-PCR) protocol was evaluated for the simultaneous detection of Potato leaf roll virus (PLRV), Potato virus X (PVX) and Potato Virus Y (PVY). The multiplex RT-PCR detection of viruses was equally efficient whether RNAs were extracted using commercial kits or a sodium sulphite based nucleic acid extraction procedure. cDNAs were prepared either using a common primer (oligo dT) or specific antisense primer followed by specific primer pairs for PCR. The multiplex RT-PCR separation of strains of PVY was accomplished by using a competitive multiplex RT-PCR (with one antisense and two sense primers). The multi virus or multistrain detection approaches described here have potential application to other crop-virus combinations.
Page(s): 587-590
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.02(4) [October 2003]

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