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Title: Use of Heavy Metals for Quantification of Rhizobia and Suppression of Bacterial Contaminants in Carrier Based Inoculants
Authors: Kumar, R P Jeevan
Jauhri, K S
Keywords: Heavy-metal;Bacterial contaminant;Inhibition;Rhizobium-inoculant
Issue Date: Oct-2003
Publisher: NISCAIR-CSIR, India
Abstract: Quality analysis of commercial legume inoculants involves determination of number of live rhizobia of appropriate species per unit weight of carrier. Conventional analysis is performed by means of plate counts of rhizobia on CRYEMA, but contaminants sometimes suppress/mask the growth of rhizobial colonies and make their counts unrealistic. Plant infection-most probable number (MPN) assay is considered to be more reliable, but is relatively expensive and time consuming. In the present study, a selective medium is devised, which permits growth of selected strains of rhizobia and at the same time refrains the growth of contaminants on plates. To inhibit nonrhizobial contaminants in carrier based inoculants, heavy metals were added in different combinations. A combination of Zn and Co at a concentration of 50 ppm each was found to inhibit bacterial contaminants without impeding the growth of rhizobial colonies on CRYEMA. However, these additions were not effective in refraining the proliferation of contaminants in charcoal-soil based rhizobial inoculants at this concentration. These metals when used in higher concentrations in charcoal-soil based inoculants, adversely affected nodulation and the dry matter yield of soybean.
Page(s): 577-582
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.02(4) [October 2003]

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