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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.02 [2003] >
IJBT Vol.02(4) [October 2003] >
| Title: | Studies on Hydantoinase Production using Pseudomonas Strain |
| Authors: | Gaikwad, B G
Nene, S
Kulkarni, B D |
| Keywords: | Pseudomonas sp.
Hydantoinase
Medium optimization
Semi-synthetic medium
D
L-phenylhydantoin
N-carbamoylphenylglycine |
| Issue Date: | Oct-2003 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Studies
were carried out using Pseudomonas sp. NCIM 2875 strain to achieve
optimum growth and
hydantoinase
activity. A medium containing glucose-NartO, with supplement of salts, yeast
extract (0.1%) and
peptone
was developed. Maximum cell (wet wt) yield and hydantoinase activity was 12.7 gl-1
and 4.63 units ( M of
N-carbamoylphenylglycine
formed/minlg wet wt cells), respectively. The activity was higher than that
obtained after
cultivation of strain on nutrient broth medium. |
| Page(s): | 571-576 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.02(4) [October 2003]
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