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IJBT Vol.02(4) [October 2003] >


Title: Studies on Hydantoinase Production using Pseudomonas Strain
Authors: Gaikwad, B G
Nene, S
Kulkarni, B D
Keywords: Pseudomonas sp.
Hydantoinase
Medium optimization
Semi-synthetic medium
D
L-phenylhydantoin
N-carbamoylphenylglycine
Issue Date: Oct-2003
Publisher: NISCAIR-CSIR, India
Abstract: Studies were carried out using Pseudomonas sp. NCIM 2875 strain to achieve optimum growth and hydantoinase activity. A medium containing glucose-NartO, with supplement of salts, yeast extract (0.1%) and peptone was developed. Maximum cell (wet wt) yield and hydantoinase activity was 12.7 gl-1 and 4.63 units (M of N-carbamoylphenylglycine formed/minlg wet wt cells), respectively. The activity was higher than that obtained after cultivation of strain on nutrient broth medium.
Page(s): 571-576
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.02(4) [October 2003]

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