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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.02 [2003] >
IJBT Vol.02(4) [October 2003] >
| Title: | Nitrogen Stress Induces Magnaporthe grisea (Hebert) Barr to Secrete Protoplast-Disrupting Proteins |
| Authors: | Rathour, R
Singh, B M
Plaha, P |
| Keywords: | Magnaporthe grisea
Nitrogen stress
Protoplast |
| Issue Date: | Oct-2003 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Experiments
were conducted to determine the effect of nitrogen stress on the gene
expression of Magnaportlze
grisea
(Hebert) Barr, a casual agent of blast disease of rice and other
graminaceous species, Concentrated culture
filtrate
extracts of the fungus grown in nitrogen deficient medium exhibited strong
disruptive activity against the
protoplasts
of different plant species including rice. However, the same activity was not
observed in culture filtrate
extracts
of the fungus grown in nitrogen rich medium. The results suggest that nitrogen
stress induces the fungus to
secrete
protoplast-disrupting factors. The nitrogen stress elicited
protoplast-disrupting activity was found to be
conserved
among different host-limited forms of the pathogen. The microscopic events
preceding the protoplast
death
and the rapidity of the response evinced plasma membrane to be the site of
action of protoplast-disrupting
factors.
Preliminary characterization of culture filtrate extracts suggested the heat
labile proteins of 20.4 to 22.4 kDa
to be the prime candidates for protoplast-disrupting
factors. |
| Page(s): | 558-563 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.02(4) [October 2003]
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