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|Title:||Nitrogen Stress Induces Magnaporthe grisea (Hebert) Barr to Secrete Protoplast-Disrupting Proteins|
Singh, B M
|Keywords:||Magnaporthe grisea;Nitrogen stress;Protoplast|
|Abstract:||Experiments were conducted to determine the effect of nitrogen stress on the gene expression of Magnaportlze grisea (Hebert) Barr, a casual agent of blast disease of rice and other graminaceous species, Concentrated culture filtrate extracts of the fungus grown in nitrogen deficient medium exhibited strong disruptive activity against the protoplasts of different plant species including rice. However, the same activity was not observed in culture filtrate extracts of the fungus grown in nitrogen rich medium. The results suggest that nitrogen stress induces the fungus to secrete protoplast-disrupting factors. The nitrogen stress elicited protoplast-disrupting activity was found to be conserved among different host-limited forms of the pathogen. The microscopic events preceding the protoplast death and the rapidity of the response evinced plasma membrane to be the site of action of protoplast-disrupting factors. Preliminary characterization of culture filtrate extracts suggested the heat labile proteins of 20.4 to 22.4 kDa to be the prime candidates for protoplast-disrupting factors.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.02(4) [October 2003]|
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