22-May-2013
15:32:00 IST
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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.02 [2003] >
IJBT Vol.02(4) [October 2003] >
| Title: | Protein Polymorphisms in Mastomys [Praomys (mastomys) coucha] |
| Authors: | Maity, B
Gupta, N
Guru, P Y |
| Keywords: | Protein polymorphisms
Allelic pattern
Cellulose acetate electrophoresis
Mastomys |
| Issue Date: | Oct-2003 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Outbred
mastomys [Praomys (mastomys) coucha], were examined at 15 chromosomal
loci encoding proteins and enzymes using cellulose acetate electrophoresis
(CAE) to establish protein polymorphisms and allelic patterns.
Protein
polymorphisms were found in five protein biochemical markers (Akp-I, Ce-2,
Idh-I, Pgm-I and Trf-I), As a result, inter species genetic variations in terms
of observed heterozygosity (Hob=0.020±O.036) and expected heterozygosity
(HE=0.136±0.205) were also observed, But polymorphic Idh-I was in very low
frequency (average af =0.487). Alb-l, Akp-l, Ce-2, Car-L, Es-2, Hbb, Idh-I,
Pgm-I, Pep-3 and Trf-I had two alleles (alb or ab), whereas Es-1, Gpi-l and
Mod-I had single allele (aIb), and C-3, Es-3, Idh-I had three alleles (abc).
The genetic variations as well
as allele frequencies within the strain may be useful
for the biomedical research for proper designing of the study protocols. This
study may also help the animal breeders to establish inbred lines of this
strain in India
on the basis of dominant/recessive alleles as per experimental requirements. |
| Page(s): | 553-557 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.02(4) [October 2003]
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