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Title: Expression of Recombinant Fatty Acid Binding Protein from Fasciola gigantica
Authors: Sriveny, D
Raina, O K
Verma, Jaya
Yadav, S C
Keywords: Fasciola gigantica
Recombinant fatty acid binding protein
Nickel chelating affinity chromatography
Issue Date: Oct-2003
Publisher: NISCAIR-CSIR, India
Abstract: Fatty acid binding protein (FADP) is an attractive vaccine candidate and a drug target for controlling fasciolosis caused by Fasciola gigantica in ruminants. The cDNA encoding F. gigantica FADP was amplified by polymerase chain reaction and expressed in Escherichia coli as a recombinant protein using a prokaryotic expression system. The protein was purified to homogeneity by nickel chelating affinity chromatography and subsequently by ion-exchange chromatography. The recombinant protein thus obtained will be assessed for immuno-prophylaxis and for its immunodiagnostic potential in ruminants agaiust F. gigantica.
Description: 549-552
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.02(4) [October 2003]

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