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Title: Expression of Recombinant Fatty Acid Binding Protein from <i>Fasciola gigantica</i>
Authors: Sriveny, D
Raina, O K
Verma, Jaya
Yadav, S C
Keywords: <i>Fasciola gigantica</i>
Recombinant fatty acid binding protein
Nickel chelating affinity chromatography
Issue Date: Oct-2003
Publisher: NISCAIR-CSIR, India
Abstract: Fatty acid binding protein (FADP) is an attractive vaccine candidate and a drug target for controlling fasciolosis caused by <i>Fasciola gigantica </i>in ruminants. The cDNA encoding <i>F. gigantica </i>FADP was amplified by polymerase chain reaction and expressed in <i>Escherichia coli </i>as a recombinant protein using a prokaryotic expression system. The protein was purified to homogeneity by nickel chelating affinity chromatography and subsequently by ion-exchange chromatography. The recombinant protein thus obtained will be assessed for immuno-prophylaxis and for its immunodiagnostic potential in ruminants agaiust <i>F. gigantica.</i>
Description: 549-552
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.02(4) [October 2003]

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