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Title: Expression of Capsid Precursor Polypeptide (PI) of Foot and Mouth Disease Virus Vaccine Strains in <i>Pichia pastoris</i>
Authors: Renji, R
Balamurugan, V
Saha, S N
Reddy, G R
Suryanarayana, V V S
Keywords: FMDV
<i>Pichia pastoris</i>
Issue Date: Oct-2003
Publisher: NISCAIR-CSIR, India
Abstract: Foot and mouth disease virus (FMDV) is the aetiological agent of a highly contagious vesicular disease of c1ovenhooved animals. The gene coding for the capsid polyprotein (P1) of FMDV from vaccine strains was amplified and cloned into the Yeast transfer vector (pPIC-9K) at <i>Eco RI </i>and <i>Not I. </i>The cloned PI gene was characterized by colony PCR, restriction enzyme digestion and sequence analysis. The recombinant plasmid of each serotype was transferred into GS1l5 strain of <i>Pichia </i>by electroporation. The <i>His<sup>+</sup> Pichia </i>transformants were anaIysed for the presence of the insert in the yeast genome by PCR. PCR positive clones of each serotype were grown separately and expression was induced with 0.5% methanol. The expressed gene product in yeast, <i>Pichia pastoris </i>was characterized by SDS-PAGE and Western Blot analysis. This is the first report on the production of FMDV structural proteins in yeast.
Description: 533-538
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.02(4) [October 2003]

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