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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.02 [2003] >
IJBT Vol.02(4) [October 2003] >
| Title: | Comparison of Lycopersicon peruvianum Mill. and L. chilense Dun. for Developement of Tomato Somatic Hybrids |
| Authors: | Patil, R S
Davey, M R
Power, J B
Cocking, E C |
| Keywords: | Tomato
Lycopersicon
L. esculentum
L. chilense
L. peruvianum
Somatic hybrid
Protoplast fusion |
| Issue Date: | Oct-2003 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Lycopersicon
peruvianum Mill. and L. chilense Dun., two species of 'Peruvianum
complex' were compared for
production
and characterisation of tomato somatic hybrids. The double selection system
"employing kanamycin
resistance
from tomato (L esculentum Mill.) cuItivar, Pusa Ruby and protoplast
regeneration pathway from wild
tomato
parents" was used for identification of heterokaryons. Somatic hybrid
plants were characterised by
morphological
markers, cytogenetic studies and biochemical analyses. The molecular
confirmation of tomato somatic
hybrids
was obtained by peR-based DNA markers. The importance of selectable marker (nptII)
gene from
cultivated species during characterisation of somatic
hybrid plants was demonstrated. |
| Page(s): | 512-523 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.02(4) [October 2003]
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