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IJBT Vol.02(4) [October 2003] >

Title: Comparison of Lycopersicon peruvianum Mill. and L. chilense Dun. for Developement of Tomato Somatic Hybrids
Authors: Patil, R S
Davey, M R
Power, J B
Cocking, E C
Keywords: Tomato
L. esculentum
L. chilense
L. peruvianum
Somatic hybrid
Protoplast fusion
Issue Date: Oct-2003
Publisher: NISCAIR-CSIR, India
Abstract: Lycopersicon peruvianum Mill. and L. chilense Dun., two species of 'Peruvianum complex' were compared for production and characterisation of tomato somatic hybrids. The double selection system "employing kanamycin resistance from tomato (L esculentum Mill.) cuItivar, Pusa Ruby and protoplast regeneration pathway from wild tomato parents" was used for identification of heterokaryons. Somatic hybrid plants were characterised by morphological markers, cytogenetic studies and biochemical analyses. The molecular confirmation of tomato somatic hybrids was obtained by peR-based DNA markers. The importance of selectable marker (nptII) gene from cultivated species during characterisation of somatic hybrid plants was demonstrated.
Page(s): 512-523
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.02(4) [October 2003]

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