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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.02 [2003] >
IJBT Vol.02(4) [October 2003] >
| Title: | Development of Long-term Cell Suspension Cultures of Wild Tomato Species, Lycopersicon chilense Dun. as Regular Source of Protoplast: An Efficient Protoplast-to-Plant System |
| Authors: | Patil, R S
Davey, M R
Power, J B
Cocking, E C |
| Keywords: | Lycopersicon chilense
Cell suspension cultures
Protoplasts
Plant regeneration |
| Issue Date: | Oct-2003 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Initiation
of direct and indirect cell suspension cultures of L. chilense Dun. was
confined to the origin of auxin in
the
culture medium. Direct cell suspensions were initiated in MSPI culture medium
containing NAA while indirect
suspensions
were initiated in culture media UM and UMI containing 2,4-D. The growth of
direct and indirect cell
suspensions
was distinguished by the growth rate and phenolic production. Direct suspensions
have shown a
continuous
growth in growth curve studies (8 day) and recorded a significantly higher cell
growth in comparison to
two
other indirect cell suspensions. In indirect suspensions, 2,4-D toxicity arose
after 5 months of culture and as a
consequence
loss of vigour followed by phenolic browning occurred. Direct cell suspensions
proved to be long-term
cultures
without any adverse effects of NAA on the growth rate and were genetically
stable. Higher protoplast yields
were
obtained from the direct cell suspension cultures which were associated with
the increased growth rate. A
productive protoplast-to-plant system was developed. |
| Page(s): | 504-511 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.02(4) [October 2003]
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