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Title: Evaluation of Yeast as an Expression System
Authors: Nasser, M W
Pooja, V
Abdin, MZ
Jain, S K
Keywords: Schizosaceharomyces pombe
Pichia pastoris
Gene expression
Issue Date: Oct-2003
Publisher: NISCAIR-CSIR, India
Abstract: Developments in recombinant DNA technology have provided an alternate route for the production of proteins. Gene expression and production of proteins of interest are of great importance for basic research as well as for biomedical applications. A number of expression systems using mammalian cells, insect cells, yeast and other bacteria as host have been developed. Yeast has received attention as a suitable host for expression of many mammalian genes due to many specific characteristics. Yeast strains, Schizosaccharomyces pombe, Saccharomyces cerevisiae and Pichia pastoris, have many advantages over other systems and may be the host of choice for the expression of complex proteins of therapeutic value. During the post-genomicera, the importance of these strains for the expression of heterologous genesmay enhance considerably.
Page(s): 477-493
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.02(4) [October 2003]

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