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IJBT Vol.02(3) [July 2003] >


Title: Production of Heterologous Microbial Lipases by Yeasts
Authors: Kademi, Ali
Lee, Byong
Houde, Alain
Keywords: Microbial lipase
Pichia pastoris
Saccharomyces cerevisiae
Expression system
Issue Date: Jul-2003
Publisher: NISCAIR-CSIR, India
Abstract: Lipases (triacylglycerol lipase, EC 3.1.1.3) catalyse the hydrolysis of triglycerides to di-glycerides, monoglycerides, glycerol and fatty acids. In non-aqueous media, these enzymes also catalyse esterification, interesteriflcation and transesterification. This versatility makes the lipases potential biocatalysts in many industrial applications. This review describes the recent advances in the use of yeasts as expression systems to produce high levels of recombinant microbial lipases. Some expression systems such as Saccharomyces cerevisiae and Pichia pastoris are reviewed and compared. Also, cell-surface display systems as novel methods to construct whole cell biocatalysts are highlighted.
Page(s): 346-355
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.02(3) [July 2003]

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