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dc.contributor.authorGupta, A K-
dc.contributor.authorAhlawat, Anita-
dc.contributor.authorBhatia, Vandanajay-
dc.identifier.issn0975-0967 (Online); 0972-5849 (Print)-
dc.description.abstractReverse transcriptase-polymerase chain reaction (RT-PCR) and RNA : DNA hybridization based diagnostic techniques were standardized and used for rapid and specific detection of equine influenza virus. RT -PCR technique based on amplification of both Non-structural-l (NS1) and Neuraminidase (NA) genes was standardized. Beside this, RNA-DNA hybridization technique using both non-structural and neuraminidase gene segments specific hot probes was also developed to directly detect this virus with out involving viral RNA isolation. Both RT-PCR and RNA-DNA hybridization techniques were successfully used for detection of influenza virus in samples within 24 to 48 hrs. These techniques can now be used as a routine tests in the laboratory for detection of influenza virus from field samples.en_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.rights CC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceIJBT Vol.02(2) [April 2003]en_US
dc.subjectEquine influenzaen_US
dc.subjectNucleic acid hybridizationen_US
dc.titleDetection of Equine Influenza Viral Genome by RT-PCR and RNA-DNA Hybridizationen_US
Appears in Collections:IJBT Vol.02(2) [April 2003]

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