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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.02 [2003] >
IJBT Vol.02(2) [April 2003] >
| Title: | Detection of Equine Influenza Viral Genome by RT-PCR and RNA-DNA Hybridization |
| Authors: | Gupta, A K
Ahlawat, Anita
Bhatia, Vandanajay |
| Keywords: | Equine influenza
RT-PCR
Nucleic acid hybridization |
| Issue Date: | Apr-2003 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Reverse
transcriptase-polymerase chain reaction (RT-PCR) and RNA : DNA hybridization
based diagnostic
techniques
were standardized and used for rapid and specific detection of equine influenza
virus. RT -PCR technique
based
on amplification of both Non-structural-l (NS1) and Neuraminidase (NA)
genes was standardized. Beside this,
RNA-DNA
hybridization technique using both non-structural and neuraminidase gene
segments specific hot probes
was
also developed to directly detect this virus with out involving viral RNA
isolation. Both RT-PCR and RNA-DNA
hybridization
techniques were successfully used for detection of influenza virus in samples
within 24 to 48 hrs. These
techniques
can now be used as a routine tests in the laboratory for detection of influenza
virus from field samples. |
| Page(s): | 214-219 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.02(2) [April 2003]
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