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Title: Detection of Equine Influenza Viral Genome by RT-PCR and RNA-DNA Hybridization
Authors: Gupta, A K
Ahlawat, Anita
Bhatia, Vandanajay
Keywords: Equine influenza;RT-PCR;Nucleic acid hybridization
Issue Date: Apr-2003
Publisher: NISCAIR-CSIR, India
Abstract: Reverse transcriptase-polymerase chain reaction (RT-PCR) and RNA : DNA hybridization based diagnostic techniques were standardized and used for rapid and specific detection of equine influenza virus. RT -PCR technique based on amplification of both Non-structural-l (NS1) and Neuraminidase (NA) genes was standardized. Beside this, RNA-DNA hybridization technique using both non-structural and neuraminidase gene segments specific hot probes was also developed to directly detect this virus with out involving viral RNA isolation. Both RT-PCR and RNA-DNA hybridization techniques were successfully used for detection of influenza virus in samples within 24 to 48 hrs. These techniques can now be used as a routine tests in the laboratory for detection of influenza virus from field samples.
Page(s): 214-219
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.02(2) [April 2003]

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