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|Title:||Detection of Equine Influenza Viral Genome by RT-PCR and RNA-DNA Hybridization|
|Authors:||Gupta, A K|
Nucleic acid hybridization
|Abstract:||Reverse transcriptase-polymerase chain reaction (RT-PCR) and RNA : DNA hybridization based diagnostic techniques were standardized and used for rapid and specific detection of equine influenza virus. RT -PCR technique based on amplification of both Non-structural-l <i>(NS1) </i>and Neuraminidase <i>(NA) </i>genes was standardized. Beside this, RNA-DNA hybridization technique using both non-structural and neuraminidase gene segments specific hot probes was also developed to directly detect this virus with out involving viral RNA isolation. Both RT-PCR and RNA-DNA hybridization techniques were successfully used for detection of influenza virus in samples within 24 to 48 hrs. These techniques can now be used as a routine tests in the laboratory for detection of influenza virus from field samples.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.02(2) [April 2003]|
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