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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.02 [2003] >
IJBT Vol.02(1) [January 2003] >
| Title: | Molecular Diagnosis and Application of DNA Markers in the Management of Fungal and Bacterial Plant Diseases |
| Authors: | Sharma, T R |
| Keywords: | Chromosome walking
DNA fingerprinting
Molecular tagging
Gene pyramiding
Resistance gene cloning |
| Issue Date: | Jan-2003 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Successful
management of plant diseases is mainly dependent on the accurate and efficient
detection of plant pathogens, amount of genetic and pathogenic variability
present in a pathogen population, development of disease resistant cultivars
and deployment of effective resistance genes in different epidemiological
regions. Beside
conventional
methods of pathogen detection and breeding resistant cultivars, recent
developments in molecular biology techniques particularly the advent of various
DNA markers have greatly influenced the plant protection methods. Pathogen
detection has relied on isolation of microorganisms and observations of
symptoms they induce on
susceptible
hosts. In many situations cultural, morphological and chemical markers have
been used to study variation in pathogen populations. Such markers are limited,
often unstable and are influenced by environmental conditions. Molecular
detection of plant pathogens and characterization of genetic variability by
using different DNA
markers have offered additional tools in the hands of
plant pathologists and plant breeders. Various PCR based and hybridization
based DNA marker techniques can be used for the characterization of genetic
variability in pathogens and molecular tagging of disease resistance genes. DNA
markers linked to specific resistance gene can be used in marker-assisted-selection
for resistance breeding, gene pyramiding and map-based cloning of the
resistance genes. In this communication various uses of DNA markers in pathogen
diagnostics and mapping, pyramiding and map-based cloning of disease resistance
genes have been discussed. |
| Page(s): | 99-109 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.02(1) [January 2003]
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