|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.02 [2003] >
IJBT Vol.02(1) [January 2003] >
| Title: | Protoplast Fusion and Brassica Improvement |
| Authors: | Kirti, P B
Prakash, S
Bhat, S R
Chopra, V L |
| Keywords: | Brassica improvement
Protoplast fusion
Cytoplasmic hybrids
Mitochondrial genome recombination
Cytoplasmic male sterility-fertility-restorer systems
Manipulation of flower morphology |
| Issue Date: | Jan-2003 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Brassica
coenospecies has been bestowed with a large collection of wild and weedy
relatives, which could act as donors of genes controlling agronomically
important traits like disease resistance, yield attributes, modified fatty acid
composition in the seed etc. Additionally, the cytoplasm of these species could
be the sources for developing alloplasmic male sterility systems that are
important in developing hybrid mustard varieties. The technology of protoplast
culture and protoplast fusion has been very well developed for crop Brassicas
and this could be effectively exploited in developing somatic hybrids and
cytoplasmic hybrids, in short called as cybrids. This could pave the way for
overcoming problems associated with the development of incompatibility barriers
and allow for obtaining hybrids of the wild species with the cultivated species
and manipulating characters that are controlled by organelle genomes. In Nicotiana
species, protoplast fusion resulted in the development of novel
mitochondrial genomes and plants carrying novel genomes have differing flower
morphology. Protoplast fusion in Cruciferae also nearly invariably
results in mitochondrial genome recombination/rearrangement. Hence, protoplast
fusion can be
effectively utilized in manipulating flower morphology
and male sterility in crop Brassicas. |
| Page(s): | 76-84 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.02(1) [January 2003]
|
|
