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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.02 [2003] >
IJBT Vol.02(1) [January 2003] >
| Title: | Biotechnological Importance of Piriformospora indica Verma et al-A Novel Symbiotic Mycorrhiza-like Fungus: An Overview |
| Authors: | Singh, Anjana
Singh, Archana
Kumari, Meera
Rai, Mahendra K
Varma, Ajit |
| Keywords: | Root colonizing
Positive influence
Economically important plants
Axenic culture
Synthetic medium |
| Issue Date: | Jan-2003 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Piriformospora indica Verma et
al, a newly discovered root colonizing, AM fungi-like fungus, showed
prominent positive influence on a wide range of plants of agriculture, forestry
and flori-horticultural importance. Interestingly, P. indica
has a wide host range of monocots and dicots including legumes, terrestrial
orchids (Dactylorhiza maculata) and members of the
bryophytes (Aneura pinguis). The fungus showed potential as
an agent for biological control of disease against soil-borne root pathogens. 32P
experiments suggest that this fungus is important for phosphorus acquisition by
the roots, especially in the arid and semi-arid regions. Mycelium could utilize
a wide variety of inorganic and organic phosphate chemicals and produced acid
phosphatases at the tip of the hyphae. The fungus was found to act as an
excellent tool for biological hardening of tissue culture raised plants (tool
for biological hardening). Fungus can be axenically grown on a wide range of
synthetic simple and complex media with sucrose or glucose as carbon energy
source. Mass cultivation of the fungus can be easily achieved on simplified
broth culture. The growth is best obtained between 25-35°C and pH
5.8. The fungus was discovered from the rhizospheric soils of desert plants, Prosopis
chilensis Stuntz and Ziziphus nummularia Burm. f.
in the sandy desert of Rajasthan, North-west India. For its characteristic spore
structure the isolate was named Piriformospora indica.
Electron microscopy revealed the presence of typical doli pore septum with
continuous parenthosomes, which indicated that the fungus belongs to the
Hymenom)'cetes (Basidiomycota). Sequences of 18S rRNA and 28S rRNA indicate
that P. indica is related to the Rhizoctonia
group and the family Sebacinaceae (Basidiomycetes).
Immunofluorescence, ELISA, western blot and immuno-gold characterization
indicated affinity of P. indica with the members of Glomeromycota,
namely Glomerales, Diversisporales and Archeaosporales. |
| Page(s): | 65-75 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.02(1) [January 2003]
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