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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.02 [2003] >
IJBT Vol.02(1) [January 2003] >
| Title: | Mass Spectrometry: An Essential Tool for Genome and Proteome Analysis |
| Authors: | Gupta, Pushpendra Kumar
Rustgi, Sachin |
| Keywords: | Mass spectrometry
Genome analysis
Proteome analysis
MALDI-TOF MS |
| Issue Date: | Jan-2003 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Mass
spectrometry (MS), in its various forms, has become an essential tool for
genome and proteome analysis. It
involves
gaseous ionization of the analyte to be examined, followed by separation of
ions according to mass-to-charge
(mlz)
ratio and determination of molecular masses of iOIlSfrom mass spectra obtained
after mass spectrometry of
analyte.
Several methods for ionization, mainly including MALDI and ES, each coupled
with a specific mass spectral
analysis
system (e.g. TOF-MS and quadrupole MS) are available. MS/MS is devised
particularly for the
determination
of amino acid sequences of small peptide. The advantage of MS over other
techniques is its speed,
since
gel electrophoresis and labeling of the analyte, needed in other techniques
used for genome/proteome analysis,
can
be dispensed with. Applications of mass spectrometry for genome analysis
include DNA sequencing and SNP
detection,
the latter involving PinPoint assay (minisequencing), PNA hybridization,
invader cleavage, "MALDI on a
chip",
etc. Similarly, its applications for proteome analysis include peptide
sequencing, determination of molecular
weights
of proteins and protein identification by database search. Protein
modifications and protein-protein
interactions
can also be examined by coupling mass spectrometry with database search. In this
manner, mass
spectrometry has become an essential tool for genome
and proteome analysis. |
| Page(s): | 48-64 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.02(1) [January 2003]
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