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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.02 [2003] >
IJBT Vol.02(1) [January 2003] >
| Title: | Agrobacterium-mediated Transformation Efficiency in Blackgram and Rice Enhanced by Multiple Copies of pTiBo542 virB and virG |
| Authors: | Balaji, V
Rajamuni, P
Sridevi, G
Veluthambi, K |
| Keywords: | Agrobacterium tumefaciens
VirG
GUS expression
Blackgram
Rice
Transformation |
| Issue Date: | Jan-2003 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Broad host-range Inc W plasm ids pBAL3 with 3'
end of virB and complete virG of pTiBoS42 and pToK47 with complete
virB and virG were introduced into Agrobacterium tumefaciens
vir helper strains harbouring a binary vector pBAL2 (Inc P). Transformation
efficiencies of these strains were evaluated by transforming the primary leaf segments
of black gram [Vigna mungo (Linn.) Hepper cv. CoS] and the
scutellum-derived calli of rice [Oryza sativa Linn. cv. Pusa Basmati 1].
Transformation was evaluated on the basis of GUS staining in stably transformed
calli. In the octo pine vir helper strain LBA4404 harbouring pBAL2, the
plasmid pBAL3 with the 3' end of virB and complete virG of
pTiBoS42 increased transformation efficiency by 141 % to 192%. The plasmid
pToK47 that carried pTiBoS42 virB in addition to virG did not
promote transformation of blackgram any further. In the L, L-succinamopine vir
helper strain EHAI0S, pBAL3 did not improve blackgram transformation. In
rice, transformation of LBA4404(pBAL2) occurred only when multiple copies of
pTiBoS42 vir genes were present. Transformation efficiencies with pBAL3
and pToK47 were 10 and 18%, respectively. While, multiple copies of 3'end of virB
and virG of pTiBoS42 harboured on a compatible replicon were
effective in blackgram, complete virB was additionally required for
efficient transformation of rice. |
| Page(s): | 138-146 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.02(1) [January 2003]
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