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|Title:||Agrobacterium-mediated Transformation Efficiency in Blackgram and Rice Enhanced by Multiple Copies of pTiBo542 virB and virG|
|Keywords:||Agrobacterium tumefaciens; VirG;GUS expression;Blackgram;Rice;Transformation|
|Abstract:||Broad host-range Inc W plasm ids pBAL3 with 3' end of virB and complete virG of pTiBoS42 and pToK47 with complete virB and virG were introduced into Agrobacterium tumefaciens vir helper strains harbouring a binary vector pBAL2 (Inc P). Transformation efficiencies of these strains were evaluated by transforming the primary leaf segments of black gram [Vigna mungo (Linn.) Hepper cv. CoS] and the scutellum-derived calli of rice [Oryza sativa Linn. cv. Pusa Basmati 1]. Transformation was evaluated on the basis of GUS staining in stably transformed calli. In the octo pine vir helper strain LBA4404 harbouring pBAL2, the plasmid pBAL3 with the 3' end of virB and complete virG of pTiBoS42 increased transformation efficiency by 141 % to 192%. The plasmid pToK47 that carried pTiBoS42 virB in addition to virG did not promote transformation of blackgram any further. In the L, L-succinamopine vir helper strain EHAI0S, pBAL3 did not improve blackgram transformation. In rice, transformation of LBA4404(pBAL2) occurred only when multiple copies of pTiBoS42 vir genes were present. Transformation efficiencies with pBAL3 and pToK47 were 10 and 18%, respectively. While, multiple copies of 3'end of virB and virG of pTiBoS42 harboured on a compatible replicon were effective in blackgram, complete virB was additionally required for efficient transformation of rice.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.02(1) [January 2003]|
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