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Title: <i>Agrobacterium-mediated </i>Transformation Efficiency in Blackgram and Rice Enhanced by Multiple Copies of pTiBo542 <i>virB </i>and <i>virG</i>
Authors: Balaji, V
Rajamuni, P
Sridevi, G
Veluthambi, K
Keywords: <i>Agrobacterium tumefaciens</i>
<i> VirG</i>
GUS expression
Issue Date: Jan-2003
Publisher: NISCAIR-CSIR, India
Abstract: Broad host-range Inc W plasm ids pBAL3 with 3' end of <i>virB </i>and complete <i>virG </i>of pTiBoS42 and pToK47 with complete <i>virB </i>and <i>virG </i>were introduced into <i>Agrobacterium tumefaciens vir </i>helper strains harbouring a binary vector pBAL2 (Inc P). Transformation efficiencies of these strains were evaluated by transforming the primary leaf segments of black gram <i>[Vigna mungo </i>(Linn.) Hepper cv. CoS] and the scutellum-derived calli of rice <i>[Oryza sativa </i>Linn. cv. Pusa Basmati 1]. Transformation was evaluated on the basis of GUS staining in stably transformed calli. In the octo pine <i>vir </i>helper strain LBA4404 harbouring pBAL2, the plasmid pBAL3 with the 3' end of <i>virB </i>and complete <i>virG </i>of pTiBoS42 increased transformation efficiency by 141 % to 192%. The plasmid pToK47 that carried pTiBoS42 <i>virB </i>in addition to <i>virG </i>did not promote transformation of blackgram any further. In the L, L-succinamopine <i>vir </i>helper strain EHAI0S, pBAL3 did not improve blackgram transformation. In rice, transformation of LBA4404(pBAL2) occurred only when multiple copies of pTiBoS42 <i>vir </i>genes were present. Transformation efficiencies with pBAL3 and pToK47 were 10 and 18%, respectively. While, multiple copies of 3'end of <i>virB </i>and <i>virG </i>of pTiBoS42 harboured on a compatible replicon were effective in blackgram, complete <i>virB </i>was additionally required for efficient transformation of rice.
Description: 138-146
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.02(1) [January 2003]

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