Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/11282
Title: Molecular Strategies for Developing Salt Tolerant Crops
Authors: Jain, Rajinder Kumar
Saini, Navinder
Jain, Sunita
Singh, Randhir
Keywords: Salt-tolerance
Stress genomics
Genetic engineering
Transformation
Molecular markers
Marker-assisted selection
Issue Date: Jan-2003
Publisher: NISCAIR-CSIR, India
Abstract: <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">Sal<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(81,="" 85,="" 62);="">i<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">nity is one of the most important abiotic stresses for agricu<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(81,="" 85,="" 62);="">lt<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">ural crops. Hig<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(81,="" 85,="" 62);="">h <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">concentrations of salts cau<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">se <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">hyperosmotic and ion<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(81,="" 85,="" 62);="">i<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">c stresses, whi<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(81,="" 85,="" 62);="">c<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">h<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">, <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">in turn, may generate secondary stresses such as oxidative stress, etc. Th<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">e <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">complexity and polygenic nature of sa<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(81,="" 85,="" 62);="">l<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">t tolera<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(81,="" 85,="" 62);="">n<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">ce trait has seriously limited <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(81,="" 85,="" 62);="">th<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">e effor<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(81,="" 85,="" 62);="">t<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">s to develop salt-tolera<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(81,="" 85,="" 62);="">n<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">t crop varieties. This paper reviews new molecular strategies that have been or can be used for the molecular dissection o<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">f <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">plant responses to salt stress, discovery of novel structural and regulatory genes involved in stress adaptation, an<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">d <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">transgenic and molecular marker strategies used for engineering salt <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">t<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">olerance in plants<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">. <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">Application of nove<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">l <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">techniques <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">s<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">uch as genom<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">e <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">sequencing, high-throughput analysis of genomic-scale expressed sequence tags (ESTs<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">), <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">DNA chips/cDNA microarray analyses, targeted or random mutagenesis, knockouts, molecular mapping and gain-o<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">f-<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">function or mutant complementation, is expected to accelerate the discovery of the new genes involved in stres<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">s <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">adaptation as well as improve understanding of stress biolog<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">y. <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">A number of stress-r<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">e<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">lated genes hav<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">e <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">been <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">c<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">h<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">a<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">racteriz<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">e<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">d including the ones that encode for important enzymes or a biochemical pathwa<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">y, <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">pa<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">r<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">ti<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">c<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">ipate <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">in s<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">ignaling pa<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">t<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">hways or <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">a<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">ct as transcriptional regulators for coordinated regulation of stress related genes. Some o<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(151,="" 153,="" 137);="">f <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">th<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">es<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">e genes h<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">a<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">ve been <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">s<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">uccessfully transferred in model plant specie<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">s <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">including <i>Arabidopsis, </i>rice <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">a<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">nd tobacco, and <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">a <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">m<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">a<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">rginal to significant improvement in <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">s<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">alt-tolerance has been report<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">e<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">d<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">. <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">In addition, molecular mark<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">e<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">r<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">s <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">can be used fo<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">r <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">linkage mapping of genes/QTLs for salinity tolerance trait<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">, <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">marker-assisted transfer and pyramiding of suc<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">h <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">QTL<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">s <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">int<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">o a<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">gronomi<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">c<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">ally d<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">e<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">sirable genotypes and/or for map-based <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">c<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">loning of genes. Application of transgenic an<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(151,="" 153,="" 137);="">d <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">m<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">o<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">le<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">c<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">ular marker rese<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">a<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">r<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">c<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">h <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">c<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">oupled with rapid gene discover<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">y v<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">ia functional genomic resear<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">c<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">h <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">i<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">n plants sha<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">l<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(151,="" 153,="" 137);="">l <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">provide effective means <span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">f<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(105,="" 108,="" 88);="">or designing salt-tolerant crops<span style="font-size: 12pt;" times="" new="" roman="" ;="" color:="" rgb(129,="" 132,="" 114);="">.</span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span>
Description: 121-137
URI: http://hdl.handle.net/123456789/11282
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.02(1) [January 2003]

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