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|Title:||Effect of -lactamase-catalyzed hydrolysis of cephalosporins on peroxynitrite-mediated nitration of serum albumin and cytochrome c|
|Authors:||Jakka, Surendar Reddy|
|Abstract:||The hydrolysis of -lactam antibiotics by -lactamases is one of the major bacterial defense systems. These enzymes generally hydrolyze a variety of antibiotics including the latest generation of cephalosporins, cephamycins and imipenem. In this paper, the effect of cephalosporins-based antibiotics on the peroxynitrite-mediated nitration of protein tyrosine is described. Although some of the antibiotics have weak inhibitory effect on the nitration reactions in the absence of -lactamase, they exhibit very strong inhibition in the presence of -lactamase. This is due to the elimination of heterocyclic thiol/thione moieties from cephalosporins by -lactamase-mediated hydrolysis. After the elimination, the thiols/thiones effectively scavenge peroxynitrite, leading to the inhibition of the nitration reactions.|
|Appears in Collections:||IJC-A Vol.50A(03-04) [March-April 2011]|
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