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Research Journals >
Indian Journal of Chemistry -Section A (IJC-A) >
IJC-A Vol.50A [2011] >
IJC-A Vol.50A(03-04) [March-April 2011] >
| Title: | Effect of  -lactamase-catalyzed hydrolysis of cephalosporins on peroxynitrite-mediated nitration of serum albumin and cytochrome c |
| Authors: | Jakka, Surendar Reddy
Mugesh, Govindasamy |
| Keywords: | Bioinorganic chemistry
Hydrolysis
Enzyme hydrolysis
Nitration
Protein nitration
Cephalosporins
Tyrosine
Lactams |
| Issue Date: | Mar-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The
hydrolysis of -lactam antibiotics by -lactamases
is one of the major bacterial defense systems. These enzymes generally
hydrolyze a variety of antibiotics including the latest generation of
cephalosporins, cephamycins and imipenem. In this paper, the effect of
cephalosporins-based antibiotics on the peroxynitrite-mediated nitration of
protein tyrosine is described. Although some of the antibiotics have weak
inhibitory effect on the nitration reactions in the absence of
-lactamase, they exhibit very strong
inhibition in the presence of -lactamase. This is due to the
elimination of heterocyclic thiol/thione moieties from cephalosporins by -lactamase-mediated
hydrolysis. After the elimination, the thiols/thiones effectively scavenge
peroxynitrite, leading to the inhibition of the nitration reactions. |
| Page(s): | 447-452 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0975(Online); 0376-4710(Print) |
| Source: | IJC-A Vol.50A(03-04) [March-April 2011]
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