NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Chemistry -Section A (IJC-A) >
IJC-A Vol.50A [2011] >
IJC-A Vol.50A(03-04) [March-April 2011] >


Title: Moving iron in ferritin: Leucine 154, a residue near Fe(III) during mineral buildup minimizes mineral dissolution
Authors: Haldar, Suranjana
Tosha, Takehiko
Theil, Elizabeth C
Keywords: Bioinorganic chemistry
Catalysis
Protein cages
Ferritin
Mineral dissolution
Iron
Biominerals
Issue Date: Mar-2011
Abstract: Ferritins, ancient protein nanocages, reversibly synthesize hydrated ferric oxide concentrates; minerals with thousands of iron atoms grow in 8 nm cavities of the 12 nm cages of plant and animal ferritins. Cells use ferritin iron for iron-protein cofactor synthesis and as a trap for reactive iron from damaged iron-proteins. Recent ferritin structural studies show the iron entry path through iron ion channels, oxidoreductase sites and nucleation channels, a distance of ~ 5 nm from one end of the cage subunits (4 -helix bundles) to the other. We now show that conserved L154, at the cavity entrance in a loop between helix 4 and a fifth short helix, slows mineral dissolution (50% mineral dissolution was >7 times faster in L154G ferritin). The effects on iron exit of leucine/glycine replacement in residue 154 at the end of iron entry path shows convergence of the iron entry and exit at L154 on the cage edge. The L154-dependent cage stabilization mechanism and the path that Fe(II) follows from the mineral surface to the ferritin protein are problems that remain unsolved in understanding the complex, eukaryotic ferritin protein cages that evolved for natural iron metabolism and are also used for imaging, nanocatalysis and nanomaterials.
Page(s): 414-419
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0975(Online); 0376-4710(Print)
Source:IJC-A Vol.50A(03-04) [March-April 2011]

Files in This Item:

File Description SizeFormat
IJCA 50A(03-04) 414-419.pdf549.81 kBAdobe PDFView/Open
 Current Page Visits: 653 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 509872 since 06-Feb-2009  Last updated on 11-Apr-2014Webmaster: nopr@niscair.res.in