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Indian Journal of Chemistry -Section A (IJC-A) >
IJC-A Vol.50A [2011] >
IJC-A Vol.50A(03-04) [March-April 2011] >
| Title: | Differences between two active forms of CO-bound soluble guanylate cyclase in the presence of activators and substrate and their populations revealed by resonance Raman spectroscopy |
| Authors: | Pal, Biswajit
Kitagawa, Teizo |
| Keywords: | Bioinorganic chemistry
Soluble guanylate cyclase
Resonance Raman spectroscopy
Enzyme activation |
| Issue Date: | Mar-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Soluble guanylate
cyclase is a dimeric (ab) enzyme catalyzing
the conversion of GTP to cyclic GMP which acts as a second messenger in
cellular signaling. It is the only known physiological receptor of NO and
binding of NO to its heme, which is covalently bound via a conserved His-b105, activates this enzyme several hundred folds
over its basal level. It is known that NO-binding causes the cleavage of Fe-His
bond. CO marginally activates sGC, and in the presence of some activator
molecules like YC-1 and BAY it activates to the same level as NO-bound sGC,
although a mechanism of this synergistic effect is hardly understood. Herein,
we present evidences for the presence of two forms of CO-bound sGC in the
presence of activators and deduce their structural differences and population
on the basis of resonance Raman spectroscopy. A mechanism for the synergetic
effect has been discussed. |
| Page(s): | 395-400 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0975(Online); 0376-4710(Print) |
| Source: | IJC-A Vol.50A(03-04) [March-April 2011]
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