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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.49 [2011] >
IJEB Vol.49(03) [March 2011] >
| Title: | Antibacterial activity of metabolite produced by Paenibacillus polymyxa strain HKA-15 against Xanthomonas campestris pv. phaseoli |
| Authors: | Mageshwaran, V
Walia, Suresh
Govindasamy, V
Annapurna, K |
| Keywords: | Antibacterial activity
Biocontrol
Paenibacillus polymyxa
Peptide antibiotic
Xanthomonas campestris pv. phaseoli |
| Issue Date: | Mar-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | An antibacterial
metabolite extracted from Paenibacillus
polymyxa HKA-15 showed strong inhibition against Xanthomonas campestris pv. phaseoli strains CP-1-1 and M-5. Minimum
inhibitory concentration (MIC) of crude extract against strains CP-1-1 and M-5
was found to be 1.7 mg/ml and 1.52 mg/ml, respectively. In UV-Vis range, the
absorption peak of crude extract was maximum at 240 nm. The compound is
resilience to wide range of temperature, pH, surfactants and organic
solvents. The complete loss of activity was observed when crude metabolite was
treated with pepsin (400 unit / ml). Characterization
of crude metabolite suggested its hydrophobic and peptide nature. Inhibition of
Xanthomonas campestris pv. phaseoli
by peptide like metabolite produced by Paenibacillus
polymyxa strain HKA-15 under in vitro
conditions showed ecological and biotechnological potential of strain HKA-15 to
control common blight disease in beans. |
| Page(s): | 229-233 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.49(03) [March 2011]
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