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Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.49 [2011] >
IJEB Vol.49(03) [March 2011] >
| Title: | Schwann cells promote neurite outgrowth of dorsal root ganglion neurons through secretion of nerve growth factor |
| Authors: | Hu, Jianguo
Zhou, Jiansheng
Li, Xingwu
Wang, , Fengchao
Lü, Hezuo |
| Keywords: | Dorsal root ganglia
Nerve growth factor
Neurite outgrowth
Schwann cells |
| Issue Date: | Mar-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The transplantation of Schwann cells (SCs) could successfully promote
axonal regeneration. This is likely to attribute to the adhesion molecules
expression and growth factors secretion of SCs. But which factor(s) play a key
role has not been precisely studied. In this study, an outgrowth assay using
dorsal root ganglia (DRG) neuron-SC co-culture system in vitro was performed. Co-culture of SCs or application of
SC-conditioned medium (CM) substantially and significantly increased DRG
neurite outgrowth. Further, nerve growth factor and NGF receptor (TrkA) mRNA were
highly expressed in Schwann cells and DRG neuron, respectively. The high
concentration of NGF protein was detected in SC-CM. When K-252a, a specific
inhibitor of NGF receptor was added, DRG neurite outgrowth was significantly
decreased in a concentration-dependent manner. These data strongly suggest that
SCs play important roles in neurite outgrowth of DRG neurons by secreted NGF |
| Page(s): | 177-182 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.49(03) [March 2011]
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