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Title: Non-genomic effect of L-triiodothyronine on calmodulin-dependent synaptosomal protein phosphorylation in adult rat cerebral cortex
Authors: Sarkar, Pradip K
Morris, Jason J
Martin, Joseph V
Keywords: Adult rat brain
Protein phosphorylation
Issue Date: Mar-2011
Publisher: NISCAIR-CSIR, India
Abstract: The present study was undertaken to examine calmodulin-dependent effect of thyroid hormones (THs) on synaptosomal protein phosphorylation in mature rat brain. Effect of L-triiodothyronine (L-T3) on <i style="">in vitro </i>protein phosphorylation was measured in a hypotonic lysate of synaptosomes prepared from adult male rat cerebral cortex, incubated in presence and absence of calcium ion (Ca<sup>2+</sup>) and calmodulin. L-T3 significantly enhanced incorporation of <sup>32</sup>P into synaptosomal proteins as compared to basal level of phosphorylation in the presence of Ca<sup>2+</sup> and calmodulin. Under these conditions, increase in protein phosphorylation was 47, 74 and 52% for 10 n<i style="">M</i>, 100 n<i style="">M</i> and 1 <img src='/image/spc_char/micro.gif' border=0><i style="">M</i> L-T3, respectively. Chelation of Ca<sup>2+</sup> using ethylene glycol-bis (2‑aminoethylether)-N, N, N’, N’-tetraacetic acid (EGTA) inhibited the effects of Ca<sup>2+</sup>/calmodulin on TH-stimulated protein phosphorylation levels. This study suggests that a high proportion of L-T3-stimulated protein phosphorylation involves Ca<sup>2+</sup>/calmodulin-dependent pathways in adult rat cerebrocortical synaptosomes.
Description: 169-176
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.49(03) [March 2011]

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