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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.49 [2011] >
IJEB Vol.49(03) [March 2011] >
| Title: | Non-genomic effect of L-triiodothyronine on calmodulin-dependent synaptosomal protein phosphorylation in adult rat cerebral cortex |
| Authors: | Sarkar, Pradip K
Morris, Jason J
Martin, Joseph V |
| Keywords: | Adult rat brain
Brain
Ca2+/calmodulin
Protein phosphorylation
L-Triiodothyronine |
| Issue Date: | Mar-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The present study
was undertaken to examine calmodulin-dependent effect of thyroid hormones (THs)
on synaptosomal protein phosphorylation in mature rat brain. Effect of
L-triiodothyronine (L-T3) on in vitro protein
phosphorylation was measured in a hypotonic lysate of synaptosomes prepared
from adult male rat cerebral cortex, incubated in presence and absence of
calcium ion (Ca2+) and calmodulin. L-T3 significantly enhanced
incorporation of 32P into synaptosomal proteins as compared to basal
level of phosphorylation in the presence of Ca2+ and calmodulin. Under
these conditions, increase in protein phosphorylation was 47, 74 and 52% for 10
nM, 100 nM and 1  M L-T3,
respectively. Chelation of Ca2+ using ethylene glycol-bis (2‑aminoethylether)-N,
N, N’, N’-tetraacetic acid (EGTA) inhibited the effects of Ca2+/calmodulin
on TH-stimulated protein phosphorylation levels. This study suggests that a
high proportion of L-T3-stimulated protein phosphorylation involves Ca2+/calmodulin-dependent
pathways in adult rat cerebrocortical synaptosomes. |
| Page(s): | 169-176 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.49(03) [March 2011]
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