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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.48 [2011] >
IJBB Vol.48(1) [February 2011] >
| Title: | Determination of free cisplatin in medium by differential pulse polarography after ultrasound and cisplatin treatment of a cancer cell culture |
| Authors: | Bernard, Vladan
Fojt, Lukáš
Škorpíková, Jiřina
Mornstein, Vojtěch |
| Keywords: | Cisplatin
Differential pulse polarography
Ovarian carcinoma cells A2780
Sonodynamic action
Ultrasound |
| Issue Date: | Feb-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The in vitro study was carried out for
detection of the cisplatin in free form and in culture medium, depending on
various
conditions of sonodynamic human ovarian cancer cells A2780 treatment by
differential pulse polarography (DPP). For sonodynamic treatment, we used
cisplatin alone and combined
cisplatin/ultrasound treatments. The
ultrasound exposure intensity of 1.0 and 2.0 W∙cm-2 in far field for incubation periods 1, 24 and 48
h was used. The parameters of DPP measurements were - 1 s drop time, 5 mV.s-1
voltage scan rate, 50 mV modulation amplitude and negative scanning
direction; platinum wire served as counter electrode and Ag|AgCl|3 M KCl as
reference electrode. The
results showed the dependence of free platinum quantities in
culture medium on incubation time and treatment protocol. We found difference in
concentration of free cisplatin between
conventional application of cisplatin and sonodynamic treatment. The sonodynamic
combined treatment of cisplatin and ultrasound field showed a higher cisplatin
content in the culture medium than cisplatin
treatment alone; a difference of 20% was observed for incubation time 48 h. The
results also showed the influence of a time sequence of ultrasound and
cytostatics in the sonodynamic treatment. The highest amount of free cisplatin
in the solution was found for primary application of cisplatin and the
subsequent ultrasound exposure. The quantity of free cisplatin increased with
time, namely for time intervals 1-24 h. There was no difference between the DPP
signal of cisplatin in reaction mixture containing cells in small quantities
and micro-filtered mixture without cells. Thus, the DPP method is suitable for
the detection and quantification of free cisplatin in the culture medium
of cell suspension. Ultrasound field can be important factor during cytostatic
therapy. |
| Page(s): | 59-62 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.48(1) [February 2011]
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