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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.48 [2011] >
IJBB Vol.48(1) [February 2011] >
| Title: | Immobilization of organophosphate hydrolase on biocompatible gelatin pads and its use in removal of organophosphate compounds and nerve agents |
| Authors: | Kanugula, Anantha KoteswaraRao
Repalle, Elisha Raju
Pandey, Jay Prakash
Sripad, Gunwar
Mitra, Chanchal Kumar
Dubey, Devender Kumar
Siddavattam, Dayananda |
| Keywords: | Organophosphates
Organophosphorus hydrolase (OPH)
Enzyme immobilization
Detoxification
Sarin, Methyl parathion
Gelatin |
| Issue Date: | Feb-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Bacterial
organophosphate hydrolases (OPH) have been shown to hydrolyze structurally
diverse group of organophosphate (OP) compounds and nerve agents. Due to broad
substrate range and unusual catalytic properties, the OPH has successfully been
used to develop eco-friendly strategies for detection and decontamination of OP
compounds. However, their usage has failed to gain necessary acceptance, due to
short half-life of the enzyme and loss of activity during process development.
In the present study, we report a simple procedure for immobilization of OPH on
biocompatible gelatin pads. The covalent coupling of OPH using glutaraldehyde
spacer has been found to dramatically improve the enzyme stability. There is no
apparent loss of OPH activity in OPH-gelatin pads stored at room temperature
for more than six months. As revealed by a number of kinetic parameters, the
catalytic properties of immobilized enzyme are found to be comparable to the
free enzyme. Further, the OPH‑gelatin pads effectively eliminate OP insecticide
methyl parathion and nerve agent sarin. |
| Page(s): | 29-34 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.48(1) [February 2011]
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