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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.49 [2011] >
IJEB Vol.49(02) [February 2011] >
| Title: | Induction of systemic resistance in different varieties of Solanum tuberosum by pure and crude elicitor treatment |
| Authors: | Bariya, Himanshu S
Thakkar, Vasudev R
Thakkar, Amit N
Subramanian, R B |
| Keywords: | Defense related enzymes
Elicitor
Hypersensitivity response
Phytophthora infestans
Solanum tuberosum |
| Issue Date: | Feb-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A 10 kD elicitor protein (infestin) produced
by Phytopthora infestans was purified and its efficacy for induction of systemic resistance
in resistant and susceptible varieties of Solanum
tuberosum was studied. Culture filtrates from
P. infestans with
and without purified elicitor (infestin) were used as elicitors to understand
the effect of purified elicitor (infestin) on development of systemic
resistance. Culture filtrate and purified elicitor (infestin) were found to
induce hypersensitive reaction on the leaves of resistant varieties, but not on
susceptible varieties after 48 h. Culture filtrate devoid of purified elicitor
(infestin) did not induce any necrotic spots even on resistant variety. Purified
elicitor (infestin) was found to induce glucose oxidase, NADPH oxidase, superoxide
dismutase, glutathione reductase, catalase and peroxidase enzymes in resistant S.
tuberosum plants, however the induction of these
enzymes was low in susceptible varieties. The oxidative enzymes were found to
induce earlier than antioxidative enzymes and there was negative correlation
between these two groups of enzymes. Levels of salicylic acid, phenylalanine
ammonia lyase (PAL),  -1, 3 glucanase and chitinase activities were also found
higher in resistant than in susceptible varieties. It was observed that
purified elicitor (infestin) was superior to crude culture filtrate, but was not
capable of inducing systemic resistance in susceptible varieties. |
| Page(s): | 151-162 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.49(02) [February 2011]
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