Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/11010
Title: Induction of systemic resistance in different varieties of <i style="">Solanum tuberosum </i>by pure and crude elicitor treatment
Authors: Bariya, Himanshu S
Thakkar, Vasudev R
Thakkar, Amit N
Subramanian, R B
Keywords: Defense related enzymes
Elicitor
Hypersensitivity response
<i style="">Phytophthora infestans</i>
<i style="">Solanum tuberosum</i>
Issue Date: Feb-2011
Publisher: NISCAIR-CSIR, India
Abstract: A 10 kD elicitor protein (infestin) produced by <i style="">Phytopthora infestans </i>was purified and its efficacy for induction of systemic resistance in resistant and susceptible varieties of <i style="">Solanum tuberosum </i>was studied. Culture filtrates from <i style="">P. infestans </i>with and without purified elicitor (infestin) were used as elicitors to understand the effect of purified elicitor (infestin) on development of systemic resistance. Culture filtrate and purified elicitor (infestin) were found to induce hypersensitive reaction on the leaves of resistant varieties, but not on susceptible varieties after 48 h. Culture filtrate devoid of purified elicitor (infestin) did not induce any necrotic spots even on resistant variety. Purified elicitor (infestin) was found to induce glucose oxidase, NADPH oxidase, superoxide dismutase, glutathione reductase, catalase and peroxidase enzymes in resistant <i style="">S. tuberosum </i>plants, however the induction of these enzymes was low in susceptible varieties. The oxidative enzymes were found to induce earlier than antioxidative enzymes and there was negative correlation between these two groups of enzymes. Levels of salicylic acid, phenylalanine ammonia lyase (PAL), <img src='/image/spc_char/beta.gif' border=0>-1, 3 glucanase and chitinase activities were also found higher in resistant than in susceptible varieties. It was observed that purified elicitor (infestin) was superior to crude culture filtrate, but was not capable of inducing systemic resistance in susceptible varieties.
Description: 151-162
URI: http://hdl.handle.net/123456789/11010
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.49(02) [February 2011]

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