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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
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Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.49 [2011] >
IJEB Vol.49(02) [February 2011] >
| Title: | Lentiviral-mediated RNAi targeting p38MAPK ameliorates high glucose-induced apoptosis in osteoblast MC3T3-E1 cell line |
| Authors: | Feng, Zhengping
Deng, Huacong
Du, Jia
Chen, Danyan
Jiang, Rong
Liang, Xiaoyan |
| Keywords: | Apoptosis
High glucose
Lentivirus
p38MAPK
MC3T3-E1 cell line
RNA interference |
| Issue Date: | Feb-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The p38 mitogen
activated protein kinase (p38MAPK) pathway is an important signaling cascade
involved in cell growth, differentiation and apoptosis. High glucose activates
p38MAPK pathway in different cells, including osteoblasts. In the present
study, role of p38MAPK in high glucose induced osteoblast apoptosis and
potential of RNA interference (RNAi) targeting p38MAPK as a therapy strategy
have been reported. Lentiviral-mediated RNAi effectively reduced p38MAPK and
p-p38MAPK expressions in osteoblastic cell line (MC3T3-E1) following high glucose
(22 mM) induction. Inhibition of p38MAPK activity
significantly suppressed high glucose induced apoptosis of MC3T3-E1 cell and
was confirmed by flow cytometry and ultra-structural examination by
transmission electronic microscope. Inhibition of p38MAPK also significantly
attenuates caspase-3 and bax protein expressions, but increased significantly
bcl-2 expression as determined by Western blot analysis. The results suggested
that p38MAPK mediates high glucose induced osteoblast apoptosis, partly through
modulating the expressions of caspase-3, bax and bcl-2. Inhibition of p38MAPK
with lentiviral-mediated RNAi or its specific inhibitor provides a new strategy
to treat high glucose induced osteoblast apoptosis. |
| Page(s): | 94-104 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.49(02) [February 2011]
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