Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/10961
Title: Rapid and efficient plant regeneration of eggplant (<i>Solanum melongena </i>L<i>.</i>) from cotyledonary leaf explants
Authors: Shivaraj, G
Rao, Srinath
Keywords: Brinjal
cotyledonary leaf
eggplant
plant regeneration
IBA
<i style="">Solanum melongena</i>
Issue Date: Jan-2011
Publisher: NISCAIR-CSIR, India
Abstract: The aim of this study was to develop an efficient protocol for establishment of plant regeneration through cotyledonary leaf explants of Eggplant (<i style="">Solanum melongena</i> L.). Two varieties of brinjal [Pusa purple long (PPL) and Black beauty (BB)] were used. High frequency and rapid regeneration protocol was developed from cotyledonary leaf explants on MS medium supplemented with either 6-Benzyl amino purine (BAP), Kinetin (Kn), Thidiazuron (TDZ) or Zeatin (Ze). The highest number of shoots (23.3±0.10) was obtained on MS medium containg 2.0 mg/BAP+0.5 mg/L Kn. The <i style="">in vitro</i> regenerated small shoots were further elongated on MS medium supplemented with gibberellic acid (GA<sub>3</sub>) at 1.5 mg/L. Elongated shoots were then excised from shoot clumps and transferred to rooting medium containg indole butyric acid (IBA) at 3.0 mg/L. The rooted plantlets were hardened on MS basal liquid medium and subsequently transferred to polycups containg vermiculate:soil:sand (1:2:2). Plantlets, thus developed were successfully established and finally transferred to a greenhouse. The plantlets showed high survival rate (80%) in the soil.
Description: 125-129
URI: http://hdl.handle.net/123456789/10961
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.10(1) [January 2011]

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