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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(1) [January 2011] >
| Title: | Rapid and efficient plant regeneration of eggplant (Solanum melongena L.) from cotyledonary leaf explants |
| Authors: | Shivaraj, G
Rao, Srinath |
| Keywords: | Brinjal
cotyledonary leaf
eggplant
plant regeneration
IBA
Solanum melongena |
| Issue Date: | Jan-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The aim of this study was to develop an
efficient protocol for establishment of plant regeneration through cotyledonary
leaf explants of Eggplant (Solanum
melongena L.). Two varieties of brinjal [Pusa purple long (PPL) and Black beauty
(BB)] were used. High frequency and rapid regeneration protocol was developed
from cotyledonary leaf explants on MS medium supplemented with either 6-Benzyl
amino purine (BAP), Kinetin (Kn), Thidiazuron (TDZ) or Zeatin (Ze). The highest
number of shoots (23.3±0.10)
was obtained on MS medium containg 2.0 mg/BAP+0.5 mg/L Kn. The in vitro regenerated small shoots were
further elongated on MS medium supplemented with gibberellic acid (GA3)
at 1.5 mg/L. Elongated shoots were then excised from shoot clumps and transferred
to rooting medium containg indole butyric acid (IBA) at 3.0 mg/L. The rooted
plantlets were hardened on MS basal liquid medium and subsequently transferred
to polycups containg vermiculate:soil:sand (1:2:2). Plantlets, thus developed
were successfully established and finally transferred to a greenhouse. The
plantlets showed high survival rate (80%) in the soil. |
| Page(s): | 125-129 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(1) [January 2011]
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