NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(1) [January 2011] >

Title: Genetic diversity of coal mine spoil by metagenomes using random amplified polymorphic DNA (RAPD) marker
Authors: Patel, Amiya Kumar
Behera, Niranjan
Keywords: Coal mine spoil
diversity index
genetic diversity
Issue Date: Jan-2011
Publisher: NISCAIR-CSIR, India
Abstract: Pit-scarred-landscape with huge heaps of coal mine spoil is the usual scenario of an open cast coal mine area. To assess the status of microbial ecosystem, genetic characterization among 18 metagenomes, sampled within six different age series coal mine spoil overburdens, were analyzed using RAPD. Among studied populations, the genetic diversity was high, having 66.21% polymorphic bands, and it showed Shannon’s information index value 0.3414. Analysis of molecular variance (AMOVA) indicated that 90% of the genetic diversity among populations was attributed to different age series overburdens, while 10% was attributed to different habitats. The existence of genetic variation, as revealed by RAPD, was coupled with bacterial colony forming units (CFUs; r =0.945), and 81.49% variability in CFU can be explained due to genetic diversity (r = 0.902). Further, the relationship between polymorphic loci and Nei’s genetic diversity index (H) was observed to be significant (r =0.876), indicating gradual enrichment in the existing genetic composition due to reclamation process with the increase in age, and mine spoil gradually starts supporting greater genetic diversity.
Page(s): 90-96
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.10(1) [January 2011]

Files in This Item:

File Description SizeFormat
IJBT 10(1) 90-96.pdf145.52 kBAdobe PDFView/Open
 Current Page Visits: 158 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 167017 since 01-Sep-2015  Last updated on 28-Jun-2016Webmaster: