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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(1) [January 2011] >
| Title: | Flux balance analysis for maximizing polyhydroxyalkanoate production in Pseudomonas putida |
| Authors: | Ramalingam, S
Vikram, M
Vigneshbabu, M P
Sivasankari, M |
| Keywords: | Biopolymers
chemostat
C/N ratio
flux balance analysis
PHA
Pseudomonas putida |
| Issue Date: | Jan-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The aim of this work is to develop process
strategies to understand the behaviour of Psendomonas putida for
cost-effective synthesis of mcl-PHA with the help of metabolic flux model
developed using linear programming. After screening and
selection from various carbon sources in shake flask experiments, 2 carbon
sources, namely, glucose and glycerol were selected for further studies. The
possibilities for higher PHA biosynthesis were illustrated by the flux balance
analysis (FBA) supplemented with continuous cultivation data. The optimal ratio
between glucose, glycerol and nitrogen is obtained by simulation of the
metabolic model. But the cost of raw materials is the tailback for the
cost-effective production of mcl-PHA. Hence, a metabolic flux distribution
model was developed for dual substrate utilization by co-feeding glucose and
glycerol for optimal PHA synthesis. Response surface results indicated that the
highest PHA accumulation conditions for glucose and glycerol. |
| Page(s): | 70-74 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(1) [January 2011]
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