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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(1) [January 2011] >
| Title: | Production, optimization and characterization of  -fructofuranosidase by Chrysonilia sitophila PSSF84—A novel source |
| Authors: | Patil, P R
Reddy, G S N
Sulochana, M B |
| Keywords: | Banana leaf
Chrysonilia sitophila PSSF84
-fructofuranosidase
fructo oligosaccharides
LCMS |
| Issue Date: | Jan-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The present work is aimed to establish optimal conditions for the
maximum production of -fructofuranosidase, which has the ability to synthesise
fructooligosacharides, important dietary constituents for human consumption,
using agro-based residues, and to identify its hydrolysis products. The maximum
-fructofuranosidase activity (34.43±0.25 U/mL) by C. sitophila PSSF84 was observed at 120 h of cultivation at pH 5.0 and 40°C in a basal medium
containing banana leaf (2%) supplemented with 0.5% peptone as the carbon and
energy source. Characterization
studies showed that the optimal pH and temperature of -fructofuranosidase
from crude extract was 5.0 and 50°C, respectively. The pH and thermo stability of enzyme was
good in the absence of any stabilizers (50% of residual activity was maintained
up to 48 h) between pH 4.5 to 5.5 and
temp 40 to 50°C. Metal ions (K, Ba, Mg, Ca, & Cu) and urea enhanced the
enzyme activity to 1.5-fold. For food industry, C. sitophila PSSF84 would be a promising strain as it can utilize
agro-waste in the production of desired biocatalyst for the synthesis of
fructooligosacharides. |
| Page(s): | 56-64 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(1) [January 2011]
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