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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(1) [January 2011] >
| Title: | Genetic diversity and bottleneck analysis of Kilakarsal sheep by microsatellite markers |
| Authors: | Radha, P
Sivaselvam, S N
Kumarasamy, P
Kumanan, K |
| Keywords: | Kilakarsal sheep
microsatellite markers
genetic variation
bottleneck analysis |
| Issue Date: | Jan-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The genetic diversity in the Kilakarsal
sheep population was analysed using 25 microsatellite markers by multiplex PCR in
five panels and automated DNA sequencer. The number of alleles ranged from 3 to
13 with a mean of 7.6 across loci.
The mean effective number of alleles was 3.88. The size and frequencies of the alleles
ranged from 80 to 278 bp and from 0.0100 to 0.8000, respectively. The PIC values
varied from 0.591 to 0.917 with a mean of 0.831. Only seven out of the
25 loci were in Hardy-Weinberg equilibrium. The mean observed and expected
heterozygosity was 0.618 and 0.725, respectively. Sixteen loci had positive FIS
values and nine had negative values. The mean FIS was 0.147. Mode-shift
analysis revealed a normal L-shaped curve indicating that Kilakarsal population
is non-bottlenecked. Results of the present study will help to formulate conservation
strategies for the Kilakarsal sheep. |
| Page(s): | 52-55 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(1) [January 2011]
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