Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/10951
Title: Genetic diversity and bottleneck analysis of Kilakarsal sheep by microsatellite markers
Authors: Radha, P
Sivaselvam, S N
Kumarasamy, P
Kumanan, K
Keywords: Kilakarsal sheep
microsatellite markers
genetic variation
bottleneck analysis
Issue Date: Jan-2011
Publisher: NISCAIR-CSIR, India
Abstract: The genetic diversity in the Kilakarsal sheep population was analysed using 25 microsatellite markers by multiplex PCR in five panels and automated DNA sequencer. The number of alleles ranged from 3 to 13 with a mean of 7.6 across loci. The mean effective number of alleles was 3.88. The size and frequencies of the alleles ranged from 80 to 278 bp and from 0.0100 to 0.8000, respectively. The PIC values varied from 0.591 to 0.917 with a mean of 0.831. Only seven out of the 25 loci were in Hardy-Weinberg equilibrium. The mean observed and expected heterozygosity was 0.618 and 0.725, respectively. Sixteen loci had positive F<sub>IS</sub> values and nine had negative values. The mean F<sub>IS</sub> was 0.147. Mode-shift analysis revealed a normal L-shaped curve indicating that Kilakarsal population is non-bottlenecked. Results of the present study will help to formulate conservation strategies for the Kilakarsal sheep.
Description: 52-55
URI: http://hdl.handle.net/123456789/10951
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.10(1) [January 2011]

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