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Title: PCR-RAPD and cross species rat microsatellite loci amplification in <i>Mastomys </i>[<i>Praomys</i>]<i> coucha </i>Smith, 1836
Authors: Gupta, Arti
Gupta, Neelima
Keywords: Molecular typing
multimammate rat
<i>Mastomyces coucha</i>
microsatellite markers
Issue Date: Jan-2011
Publisher: NISCAIR-CSIR, India
Abstract: Ten RAPD primers and nine microsatellite markers of <i style="">Mastomys huberti</i> were cross tested to establish molecular characters as well as identify microsatellite markers in the genome of <i style="">Mastomys (Praomys) coucha</i>. A total of 484 bands were scored against 10 isolates of <i style="">M. coucha</i>. The per cent polymorphism was 1.66 in individual isolates but this polymorphism was not statistically significant among the 10 isolates (Mas1 to 10). Mean difference, Mas6 vs. Mas10 = 0.005, Mas5 vs. Mas3 = 0.485 and Mas7 vs. Mas9 = 0.260 were calculated by Newman-Keuls multiple comparison test using one way ANOVA among <i style="">Mastomys</i> strains. The dendogram also revealed Single linkage euclidean distances among the 10 isolates (Mas3 vs. Mas1 = 1.3, Mas8 vs. Mas5 = 1.5 and Mas9 vs. Mas5 = 1.1) but the Single linkage euclidean distances were statistically not significant (P>0.05). Identifying RG-1, 3, 4, 7 and 8 RAPD markers were amplified in all the isolates with 1200 bp, 1200 and 200 bp; 400 bp; 1000 and 700 bp and 300 bp, respectively along with other polymorphic alleles. Out of 9 microsatellite markers, 5 (MH028 - 434 bp; MH30 - 249 bp; MH105 - 271 bp; MH133 - 338 bp and MH174 - 316 bp, respectively) were exactly amplified in the genome of <i style="">M. coucha</i>. RG-1, 3, 4, 7 and 8 RAPD primers and 5 microsatellite markers may assist in the identification of <i style="">M. coucha</i>.
Description: 45-51
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.10(1) [January 2011]

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