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IJBT Vol.10 [2011] >
IJBT Vol.10(1) [January 2011] >
| Title: | PCR-RAPD and cross species rat microsatellite loci amplification in Mastomys [Praomys] coucha Smith, 1836 |
| Authors: | Gupta, Arti
Gupta, Neelima |
| Keywords: | Molecular typing
multimammate rat
muridae
Mastomyces coucha
RAPD
microsatellite markers |
| Issue Date: | Jan-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Ten RAPD primers and
nine microsatellite markers of Mastomys
huberti were cross tested to establish molecular characters as well as
identify microsatellite markers in the genome of Mastomys (Praomys) coucha. A total of 484 bands were scored against
10 isolates of M. coucha. The per
cent polymorphism was 1.66 in individual isolates but
this polymorphism was not statistically significant among the 10 isolates (Mas1
to 10). Mean difference, Mas6 vs.
Mas10 = 0.005, Mas5 vs. Mas3 = 0.485 and Mas7 vs. Mas9 = 0.260 were calculated
by Newman-Keuls multiple comparison test using one way ANOVA among Mastomys strains. The dendogram also
revealed Single linkage euclidean distances among the 10 isolates (Mas3 vs.
Mas1 = 1.3, Mas8 vs. Mas5 = 1.5 and Mas9 vs. Mas5 = 1.1) but the Single linkage
euclidean distances were statistically not significant (P>0.05). Identifying
RG-1, 3, 4, 7 and 8 RAPD markers were amplified in all the isolates with 1200
bp, 1200 and 200 bp; 400 bp; 1000 and 700 bp and 300 bp, respectively along
with other polymorphic alleles. Out of 9 microsatellite markers, 5 (MH028 - 434
bp; MH30 - 249 bp; MH105 - 271 bp; MH133 -
338 bp and MH174 - 316 bp, respectively) were exactly amplified in the genome
of M. coucha. RG-1, 3, 4, 7 and
8 RAPD primers and 5 microsatellite markers may assist in the identification
of M. coucha. |
| Page(s): | 45-51 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(1) [January 2011]
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