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IJBT Vol.10(1) [January 2011] >


Title: PCR-RAPD and cross species rat microsatellite loci amplification in Mastomys [Praomys] coucha Smith, 1836
Authors: Gupta, Arti
Gupta, Neelima
Keywords: Molecular typing
multimammate rat
muridae
Mastomyces coucha
RAPD
microsatellite markers
Issue Date: Jan-2011
Publisher: NISCAIR-CSIR, India
Abstract: Ten RAPD primers and nine microsatellite markers of Mastomys huberti were cross tested to establish molecular characters as well as identify microsatellite markers in the genome of Mastomys (Praomys) coucha. A total of 484 bands were scored against 10 isolates of M. coucha. The per cent polymorphism was 1.66 in individual isolates but this polymorphism was not statistically significant among the 10 isolates (Mas1 to 10). Mean difference, Mas6 vs. Mas10 = 0.005, Mas5 vs. Mas3 = 0.485 and Mas7 vs. Mas9 = 0.260 were calculated by Newman-Keuls multiple comparison test using one way ANOVA among Mastomys strains. The dendogram also revealed Single linkage euclidean distances among the 10 isolates (Mas3 vs. Mas1 = 1.3, Mas8 vs. Mas5 = 1.5 and Mas9 vs. Mas5 = 1.1) but the Single linkage euclidean distances were statistically not significant (P>0.05). Identifying RG-1, 3, 4, 7 and 8 RAPD markers were amplified in all the isolates with 1200 bp, 1200 and 200 bp; 400 bp; 1000 and 700 bp and 300 bp, respectively along with other polymorphic alleles. Out of 9 microsatellite markers, 5 (MH028 - 434 bp; MH30 - 249 bp; MH105 - 271 bp; MH133 - 338 bp and MH174 - 316 bp, respectively) were exactly amplified in the genome of M. coucha. RG-1, 3, 4, 7 and 8 RAPD primers and 5 microsatellite markers may assist in the identification of M. coucha.
Page(s): 45-51
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.10(1) [January 2011]

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