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IJBT Vol.10(1) [January 2011] >


Title: Comparative evaluation of whole genome amplification methods in water buffalo frozen semen
Authors: Jain, Neeraj
Muraleedharan, Padeeri
Rao, Gangadhar
Reddy, Chandrasekhar
Reddy, Sunil Kumar
Keywords: whole genome amplification
multiple displacement amplification
OmniPlex amplification
RFLP
sequencing
microsatellite genotyping
water buffalo
semen
Issue Date: Jan-2011
Publisher: NISCAIR-CSIR, India
Abstract: Whole genome amplification (WGA) provides a large amount of genomic DNA for various genetic analyses from depleting stock or scarce sources of DNA. In the present study, the performance of two routinely used WGA methods i.e., Multiple displacement amplification (MDA) and OmniPlex amplification was compared against the unamplified semen DNA of 9 water buffalo bulls by PCR, RFLP, sequencing and microsatellite genotyping. Using wgaDNA, no difference was seen in RFLP or sequencing results by either method. However, 2.2% erroneous microsatellite genotype calls were observed in OmniPlex amplification compared to 0.6% by MDA technique. Though, the two whole genome amplification methods provide large amount of template DNA required for applications such as genome mapping, a careful selection of methodologies would be required in view of inconsistency in genotyping results.
Page(s): 39-44
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.10(1) [January 2011]

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