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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(1) [January 2011] >
| Title: | Comparative evaluation of whole genome amplification methods in water buffalo frozen semen |
| Authors: | Jain, Neeraj
Muraleedharan, Padeeri
Rao, Gangadhar
Reddy, Chandrasekhar
Reddy, Sunil Kumar |
| Keywords: | whole genome amplification
multiple displacement amplification
OmniPlex amplification
RFLP
sequencing
microsatellite genotyping
water buffalo
semen |
| Issue Date: | Jan-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Whole genome
amplification (WGA) provides a large amount of genomic DNA for various genetic
analyses from depleting stock or scarce sources of DNA. In the present study,
the performance of two routinely used WGA methods i.e., Multiple displacement
amplification (MDA) and OmniPlex amplification was compared against the
unamplified semen DNA of 9 water buffalo bulls by PCR, RFLP, sequencing and
microsatellite genotyping. Using wgaDNA, no difference was seen in RFLP or
sequencing results by either method. However, 2.2% erroneous microsatellite
genotype calls were observed in OmniPlex amplification compared to 0.6% by MDA
technique. Though, the two whole genome amplification methods provide large
amount of template DNA required for applications such as genome mapping, a
careful selection of methodologies would be required in view of inconsistency
in genotyping results. |
| Page(s): | 39-44 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(1) [January 2011]
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