|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(1) [January 2011] >
| Title: | Agrobacterium-mediated transformation of eggplant (Solanum melongena L.) using cotyledon explants and coat protein gene of Cucumber mosaic virus |
| Authors: | Pratap, Dharmendra
Kumar, Susheel
Raj, Shri Krishna
Sharma, Ashok Kumar |
| Keywords: | Agrobacterium tumefaciens
coat protein gene
cotyledon explants
Cucumber mosaic virus
eggplant
genetic transformation
Solanum melongena |
| Issue Date: | Jan-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Genetic transformation of eggplant (Solanum melongena L.)
was attempted using
cotyledon explants and Agrobacterium tumefaciens
(strain LBA4404) carrying Cucumber mosaic virus (CMV) coat protein (CP)
gene construct under the control of CaMV 35S promoter in pROK2 binary vector. A total of 110 cotyledon explants were employed in 4
independent co-cultivation events, of which 66 putative transgenic plants were
established in glasshouse. The
integration of
CMV-CP transgene in the genome of transgenic plants was confirmed
by polymerase chain reaction (PCR) using CMV-CP specific primers. Southern
hybridization tests with CMV-CP specific probe confirmed the insertion of
single or double copy number of transgene in their genome, which successfully
transcribed and translated 26 kDa protein. The regenerated transgenic plants
were self-pollinated and grown under glasshouse conditions. T0 generation
transgenic plants showed a significant degree of resistance against CMV when
screened by challenge inoculations. |
| Page(s): | 19-24 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(1) [January 2011]
|
|
