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Title: <i style="">Agrobacterium</i>-mediated transformation of eggplant (<i style="">Solanum melongena</i> L.) using cotyledon explants and coat protein gene of <i style="">Cucumber mosaic virus</i>
Authors: Pratap, Dharmendra
Kumar, Susheel
Raj, Shri Krishna
Sharma, Ashok Kumar
Keywords: <i style="">Agrobacterium tumefaciens</i>
coat protein gene
cotyledon explants
<i style="">Cucumber mosaic virus</i>
genetic transformation
<i>Solanum melongena</i>
Issue Date: Jan-2011
Publisher: NISCAIR-CSIR, India
Abstract: Genetic transformation of eggplant (<i>Solanum melongena</i> L.) was attempted using cotyledon explants and <i>Agrobacterium</i> <i style="">tumefaciens</i> (strain LBA4404) carrying <i>Cucumber mosaic virus</i> (CMV) coat protein (CP) gene construct under the control of CaMV 35S promoter in p<i style="">ROK</i>2 binary vector.<i style=""> </i>A total of 110 cotyledon explants were employed in 4 independent co-cultivation events, of which 66 putative transgenic plants were established in glasshouse. The integration of CMV-CP transgene in the genome of transgenic plants was confirmed by polymerase chain reaction (PCR) using CMV-CP specific primers. Southern hybridization tests with CMV-CP specific probe confirmed the insertion of single or double copy number of transgene in their genome, which successfully transcribed and translated 26 kDa protein. The regenerated transgenic plants were self-pollinated and grown under glasshouse conditions. T<sub>0 &nbsp;</sub>generation transgenic plants showed a significant degree of resistance against CMV when screened by challenge inoculations.
Description: 19-24
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.10(1) [January 2011]

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