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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.44 [2007] >
IJBB Vol.44(3) [June 2007] >
| Title: | Triiodothyronine and melatonin influence antioxidant defense mechanism in a teleost Anabas testudineus (Bloch): In vitro study |
| Authors: | Sreejith, P
Beyo, R S
Divya, L
Vijayasree, A S
Manju, M
Oommen, O V |
| Keywords: | Anabas testudineus
Antioxidant enzyme
Fish
Free radicals
Lipid peroxidation
Melatonin
Photoperiod
6-Propyl thiouracil
Triiodothyronine |
| Issue Date: | Jun-2007 |
| Publisher: | CSIR |
| Abstract: | The effect of the hormones triiodothyronine (T₃) and melatonin on antioxidant defense system was studied in 6-propyl thiouracil (6-PTU)-treated or photoperiod-exposed teleost Anabas testudineus. 6-PTU (2 µg/g) treatment or photoperiod exposure (24 h) increased malondialdehyde (MDA) and conjugated dienes (CD) concentrations, indicating increased lipid peroxidation (LPO) in the experimental conditions. T₃ or melatonin (10⁻⁶ M) treatment for 15 min in vitro in PTU-treated fish reversed the activity of superoxide dismutase (SOD), catalase and glutathione content. T₃ -treated group showed no change in glutathione peroxidase (GPx) activity, whereas melatonin treatment decreased its activity. T₃ inhibited glutathione reductase (GR) activity. Photoperiod exposure (physiological pinealotomy) induced a stressful situation in this teleost, as evidenced by LPO products and antioxidant enzyme activities. Melatonin and T₃ treatment for 15 min in vitro also reversed the effect of photoperiod on peroxidation products and the SOD and catalase activities. GR activity decreased in photoperiod-exposed group and melatonin and T₃ treatment reversed the activities. The antioxidant enzymes responded to the stress situation after 6-PTU treatment and photoperiod exposure by altering their activities. The study suggested an independent effect of T₃ and melatonin on antioxidant defence mechanism in different physiological situations in fish. |
| Page(s): | 164-168 |
| ISSN: | 0301-1208 |
| Source: | IJBB Vol.44(3) [June 2007]
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