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Title: | Triiodothyronine and melatonin influence antioxidant defense mechanism in a teleost Anabas testudineus (Bloch): In vitro study |
Authors: | Sreejith, P Beyo, R S Divya, L Vijayasree, A S Manju, M Oommen, O V |
Keywords: | Anabas testudineus;Antioxidant enzyme;Fish;Free radicals;Lipid peroxidation;Melatonin;Photoperiod;6-Propyl thiouracil;Triiodothyronine |
Issue Date: | Jun-2007 |
Publisher: | CSIR |
Abstract: | The effect of the hormones triiodothyronine (T₃) and melatonin on antioxidant defense system was studied in 6-propyl thiouracil (6-PTU)-treated or photoperiod-exposed teleost Anabas testudineus. 6-PTU (2 µg/g) treatment or photoperiod exposure (24 h) increased malondialdehyde (MDA) and conjugated dienes (CD) concentrations, indicating increased lipid peroxidation (LPO) in the experimental conditions. T₃ or melatonin (10⁻⁶ M) treatment for 15 min in vitro in PTU-treated fish reversed the activity of superoxide dismutase (SOD), catalase and glutathione content. T₃ -treated group showed no change in glutathione peroxidase (GPx) activity, whereas melatonin treatment decreased its activity. T₃ inhibited glutathione reductase (GR) activity. Photoperiod exposure (physiological pinealotomy) induced a stressful situation in this teleost, as evidenced by LPO products and antioxidant enzyme activities. Melatonin and T₃ treatment for 15 min in vitro also reversed the effect of photoperiod on peroxidation products and the SOD and catalase activities. GR activity decreased in photoperiod-exposed group and melatonin and T₃ treatment reversed the activities. The antioxidant enzymes responded to the stress situation after 6-PTU treatment and photoperiod exposure by altering their activities. The study suggested an independent effect of T₃ and melatonin on antioxidant defence mechanism in different physiological situations in fish. |
Page(s): | 164-168 |
URI: | http://hdl.handle.net/123456789/109 |
ISSN: | 0301-1208 |
Appears in Collections: | IJBB Vol.44(3) [June 2007] |
Files in This Item:
File | Description | Size | Format | |
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IJBB 44(3) (2007) 164-168.pdf | 90.98 kB | Adobe PDF | View/Open |
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